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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
recombinant rodent p27Kip1 protein.
- 亚型:
Mouse IgG1
- 形态:
液态
- 保存条件:
保存于-20℃
- 克隆性:
单克隆
- 标记物:
无标记(PLlabs可供应生物素、酶、荧光素标记的抗体)
- 适应物种:
Hu,Mo,Rat
- 保质期:
按说明书条件可保存1-2年
- 抗原来源:
见详细说明资料
- 目录编号:
PL0401274
- 级别:
超纯
- 库存:
大量
- 供应商:
华拓生物
- 宿主:
Mouse
- 应用范围:
WB: 1:200-500
- 浓度:
1mg/ml
- 靶点:
P27(kip1)
- 抗体英文名:
P27(kip1) Antibody
- 抗体名:
P27(kip1)单克隆抗体
- 规格:
0.1ml
Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)
Background:
This product for research use only, not for use in humans, therapeutic or diagnostic procedures.
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文献和实验Procedure Dilute p27 monoclonal antibody 1:1000 (v/v) in Carbonate Coating Buffer. Add 100 µl/well and incubate o/n @ 4°C or 1 hr @37°C. Wash 3X with TBST (pour onto plate, empty into sink, hit onto towel 3x to clear wells
-induced responses, including a down-regulation of the cyclin-dependent kinase (Cdk2) inhibitors p21CIP1 and p27KIP1 , while attachment to P-L-L did not. We conclude that engagement of the FN-receptor integrin α5β1 induces integrin signaling regulating
for determining the activity of PNAs designed to target oncomiRNAs, using as model system miR-221 and its target p27Kip1 mRNA. The effects of PNAs targeting miR-221 are here presented discussing data obtained using as model system the human breast cancer cell line
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