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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1000
- 供应商:
上海沪震
- 检测范围:
见说明书
- 检测方法:
酶联免疫
- 应用:
科研
- 标记物:
见说明书
- 样本:
血清/组织/尿液
Human CAM (Calmodulin) ELISA Kit
| 英文名称 | Human CAM (Calmodulin) ELISA Kit | ||
| 中文名称 | 人钙调素(CAM)酶联免疫吸附测定试剂盒 | ||
| 货号 | 种属 | Human/人 | |
| 规格 | 96T/Kit (8*12 strips) 48T/Kit (8*6 strips) | ||
| 检测方法 | 双抗体夹心法 | ||
| 检测范围 | 93.75~6000pg/mL | 灵敏度 | 56.25pg/mL |
| 中文名称 | 英文名称 | 规格 | 保存 |
| ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8×12 / 8×6* | 4℃/-20℃ # |
| 冻干标准品 | Reference Standard | 2/1 支* | 4℃/-20℃ # |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20mL/12mL* | 4℃ |
| 浓缩生物素化抗体 | Concentrated Biotinylated Detection Ab | 1支 120μL/70μL* | 4℃/-20℃ # |
| 生物素化抗体稀释液 | Biotinytated Detection Ab Diluent | 1瓶 10mL/6mL* | 4℃ |
| 浓缩HRP酶结合物 | Concentrated HRP Conjugate | 1支 120μL/70μL* | 4℃(避光) |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 10mL/6mL* | 4℃ |
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30mL/16mL* | 4℃ |
| 底物溶液(TMB) | Substrate Reagent | 1瓶 10mL/6mL* | 4℃(避光) |
| 反应终止液 | Stop Solution | 1瓶 10mL/6mL* | 4℃ |
| 封板覆膜 | Plate Sealer | 5/3 张* | |
| 产品说明书 | Product Description | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 | |
| 特别说明: *: [96T/48T](打开包装后请及时检查所有物品是否齐全完整) #: 一周内使用可存于4℃,需长时间存放或多次使用建议存于-20℃. |
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1. 在各孔中加入标准品或样品各100μL, 37℃孵育90分钟
2. 加入100μL 生物素化抗体工作液,37℃孵育60分钟
3. 洗涤3次
4. 加入100μL酶结合物工作液, 37℃孵育30分钟
5. 洗涤5次
6. 加入90μL底物溶液, 37℃孵育15分钟左右
7. 加入50μL终止液,立即在450nm波长处测量OD值
8. 结果计算
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文献和实验Studies of Calmodulin-Dependent Regulation
Methods are presented for purifying bovine testes calmodulin and the calmodulin-regulated plasma-membrane calcium ATPase from human erythrocytes by calcium dependent affinity chromatography. The assay of CaM Kinase II using a synthetic
A Microtiter-Plate Assay of Human NOS Isoforms
isozymes are highly regulated by Ca2+ and calmodulin (CaM), whereas the iNOS has CaM tightly bound. NOS are heme proteins that also contain tightly bound flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) and require tetrahydrobiopterin (BH
Cloning and Expression of Human Inducible Nitric Oxide Synthase
was then inserted back into oocytes to confirm functional expression. Nitrite production did not require the presence of Ca2+ and was not inhibited by trifluoperazine, a calmodulin (CaM), and brain-NOS inhibitor.
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