- ¥1 - 3980
- PEPROTECH
- 中国/美国
- xy-021Mu01
- 2026年03月31日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Ferritin, Heavy Polypeptide (FTH)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Mus musculus (Mouse) |
| Product No. | xy021Mu01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 22.6kDa |
| Concentration | 5.9 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
MGHHHHHHSGSEF-MTTASPSQVR QNYHQDAEAA INRQINLELY ASYVYLSMSC YFDRDDVALK NFAKYFLHQS HEEREHAEKL MKLQNQRGGR IFLQDIKKPD RDDWESGLNA MECALHLEKS VNQSLLELHK LATDKNDPHL CDFIETYYLS EQVKSIKELG DHVTNLRKMG APEAGMAEYL FDKHTLGHGD ES
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
重肽铁蛋白(FTH)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验。 ⑸UltraCHOTM培养基 UltraCHOTM培养基最适于培养转染和未转染的中国仓鼠卵巢细胞（CHO）细胞，表达重姐蛋白质。UltraCHOTM培养基以一种改良的DMEM培养基（F-12）为基础，在此基础上添加胰岛素，转铁蛋白以及专利性的纯化蛋白等成分而成。有UltraCHO液体培养基（1X）和干粉培养基（含冷冻添加剂）两种类型的产品可供选择，培养基蛋白含量低于300ug/ml. 应用：培养CHO细胞; 重组蛋白的合成。 ⑹ProCHOTMCDM系统 ProCHO限定培养
鼠淋巴细胞的第二信号系统的研究。 ⑸UltraCHOTM培养基 UltraCHOTM培养基最适于培养转染和未转染的中国仓鼠卵巢细胞(CHO)细胞，表达重姐蛋白质。UltraCHOTM培养基以一种改良的DMEM培养基(F-12)为基础，在此基础上添加胰岛素，转铁蛋白以及专利性的纯化蛋白等成分而成。有UltraCHO液体培养基(1X)和干粉培养基(含冷冻添加剂)两种类型的产品可供选择，培养基蛋白含量低于300ug/ml. 应用：培养CHO细胞; 重组蛋白的合成
单抗多为IgG，来源主要是腹水和混合瘤培养上清夜。腹水有大量白蛋白、转铁蛋白和宿主抗体等。Protein G和ProteinA对IgG的Fc区有专一性亲和作用，能一步醇化各种不同源的IgG。血清互补剂如小牛血清可先用蛋白G预处理，在培养前除去IgG。重组蛋白A介质rProtein A Sepharose FF对IgG有更高的栽量和专一性，基团脱落更少。脱落的rProtein A用离子交换Q Sepharose Hp或凝胶过滤Superdex 200，很容易去除。 疏水层析pheny
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