- ¥1 - 3980
- PEPROTECH
- 中国/美国
- xy-827Ra01
- 2026年02月09日
14 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Netrin 1 (Ntn1)
- 库存：
1000
- 供应商：
上海信裕
轴突生长诱向因子1(Ntn1)重组蛋白
Recombinant Netrin 1 (Ntn1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy827Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 60.5kDa |
| Concentration | 8.9 |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
轴突生长诱向因子1(Ntn1)重组蛋白
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- PECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSMEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIAXKCPKIK PLKKYLLLGN AEDSP
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- PECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS GRKSGGVCLN CRHNTAGRHC HYCKEGFYRD MGKPITHRKA CKACDCHPVG AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSMEEPE DCDSYCKASK GKLKMNMKKY CRKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR GDQSLWIRSR DIAXKCPKIK PLKKYLLLGN AEDSP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
轴突生长诱向因子1(Ntn1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
轴突生长诱向因子1(Ntn1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
轴突生长诱向因子1(Ntn1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
【转贴】 从纯化到星辰---冷泉港(CSHL)蛋白质纯化课侧记（全文完）后附全文doc文档
，这几年又尽心尽 力为冷泉港筹 款，贡献巨大。 第二位教员是来自UCLA 的Albert Courey 教授。这位教授人很nice也很幽默，四位老师 中我对他印象 最好。申请这个课前，我先跟他发过e-mail 询问这个课的情况，而他也鼓励我申请。这老 哥研究果蝇的, 但是他却负责教一组纯化转录因子的实验。我一开始不太理解，后来问他的学术经历，才 明白是怎么回事。 原来这老哥博士是做生化，博后跟的是robert Tjian （钱泽南），还是做生化，研究转录 因子。后来做
基和下游工艺的相关杂质，采用适当的技术和方法进行分析检测。 六、经修饰的单克隆抗体 为了提高单克隆抗体在治疗和体内诊断中的作用，常用单抗与毒素、药物、放射性核素或其它物质偶连形成免疫结合物，或在同一段多胎链包含非免疫球蛋白和免疫球蛋白序列的嵌合重组蛋白来获得。研究者除对前面提到的有关未偶连单克隆 抗体（未修饰单克隆 抗体）的要求外，还应提供下列内容： （一）免疫结合物的构建 应提供构建免疫结合物所用试剂和过程的详细资料，包括： 1、描述与单克隆抗体连接的成分如：毒素、药物、酶及细胞因子，包括
技术资料暂无技术资料 索取技术资料
轴突生长诱向因子1(Ntn1)重组蛋白
¥1 - 3980
