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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C,避光保存
- 库存:
100
- 供应商:
远慕生物
- 规格:
20mg/1g/50mg
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文献和实验Sucrose Density Gradient Fractionation
Grow a 2 ml culture,24 hr.at 30℃ in selective mediaWhen culture is ready,use it to inoculate about 55 ml (50 ml plus 5 for O.D.600 readings)of selective media.Grow to mid-logarithmic phase (O.D.600 is about 1).This usually takes 10 hours (for 1ml
Sucrose Density Gradient Fractionation of Yeast Membranes
of growth) Wait 1 doubling period (about 2 hours) until O.D.600 equals about 1 Add 1 ml of COLD 1M NaN3 to 100 ml culture (we want 10 mM total conc.) Spin down 3 x 109 cells (about 100 O.D. equivalents (100 ml of OD = 1): 2750 X g
about 55 ml (50 ml plus 5 for O.D.600 readings) of selective media. Grow to mid-logarithmic phase (O.D.600 is about 1). This usually takes 10 hours (for 1ml of the 24 hr. culture into 50 ml media). It is helpful to inoculate 2 different flasks
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