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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1)
- 库存:
1000
- 供应商:
上海信裕
| Organism species | Rattus norvegicus (Rat) |
| Product No. | xy554Ra01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 32.8KDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
RQKVVITKVV MHPCKVLELQ MRKRGFTMGI GQYIFVNCPS ISFLEWHPFT LTSAPEEEFF SIHIRAAGDW TENLIRTFEQ QHSPMPRIEV DGPFGTVSED VFQYEVAVLV GAGIGVTPFA SFLKSIWYKF QRAHNKLKTQ KIYFYWICRE TGAFAWFNNL LNSLEQEMDE LGKPDFLNYR LFLTGWDSNI AGHAALNFDR ATDVLTGLKQ KTSFGRPMWD NEFSRIATAH PKSVVGVFLC GPPTLAKSLR KCCR
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
尼克酰胺腺嘌呤二核苷酸磷酸氧化酶1(NOX1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验微粒体酶的唯一电子供体,通过还原型烟酰胺腺嘌呤二核苷酸磷酸(reduced nicotinamide adenine dinucleotide phosphate,NADPH)将电子传递给CYP450酶,CYP450酶得到电子后再与底物发生氧化还原反应,从而发挥代谢活性。 肝微粒体中包含了大部分Ⅰ相酶,其中最重要的是以CYP450为主要成分的微粒体混合功能氧化酶系统,在用肝微粒体进行研究时,如加入相应的辅助因子NADPH,则可重组体外代谢体系,从而通过体外温孵法进行Ⅰ相代谢稳定性研究。 03
Pastris3种。以Pichia Pastoris应用最多。 甲醇酵母的表达载体为整合型质粒,载体中含有与酵母染色体中同源的序列,因而比较容易整合入酵母染色体中。大部分甲醇酵母的表达载体中都含有甲醇酵母醇氧化酶基因一1(A0x1),在该基因的启动子(PAXOI)作用下,外源基因得以表达。PAXOI是一个强启动子,在以葡萄糖或甘油为碳源时。甲醇酵母中AOx1基因的表达受到抑制,而在以甲醇为唯一碳源时PAXOI可被诱导激活,因而外源基因可在其控制下表达,将目的基因多拷贝整合入酵母染色体后可以提高
Pastris3种。以Pichia Pastoris应用最多。 甲醇酵母的表达载体为整合型质粒,载体中含有与酵母染色体中同源的序列,因而比较容易整合入酵母染色体中。大部分甲醇酵母的表达载体中都含有甲醇酵母醇氧化酶基因一1(A0x1),在该基因的启动子(PAXOI)作用下,外源基因得以表达。PAXOI是一个强启动子,在以葡萄糖或甘油为碳源时。甲醇酵母中AOx1基因的表达受到抑制,而在以甲醇为唯一碳源时PAXOI可被诱导激活,因而外源基因可在其控制下表达,将目的基因多拷贝整合入酵母染色体后可以提高
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