- 询价
- PEPROTECH
- 中国/美国
- xy-471Rb01
- 2026年01月20日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Osteocalcin (OC)
- 库存：
1000
- 供应商：
上海信裕
| Organism species | Oryctolagus cuniculus (Rabbit) |
| Product No. | xy471Rb01 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 38.4kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Lys24~Val100 with two N-terminal Tags, His-tag and GST-tag |
| Formulation | Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. |
KPSGAES GRGSAFVSKR EGSEVVKRAR RQLIDGQGAP APYPDPLEPK REVCELNPDC DELADQVGLQ DAYQRFYGPV
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
骨钙素(OC)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验the reconstituted vial to sit at 4oC for at > 2 hours before use. (将重悬液在4oC静置2小时以上) 3. This solution can be stored at 2-8oC for up to 1 week. 第 3 步：该重悬液在 2-8oC 最长可保存 1 周。 用说明书上推荐的溶液将细胞因子或重组蛋白重悬到推荐的浓度后，细胞因子或重组蛋白可放置在2-8o C，即冰箱的冷藏室。在这种条件下，细胞因子或重组蛋白的活性
)。这种情况下，您最好能将要溶解的细胞因子或重组蛋白放在水平摇床(shaker)上低速摇一段时间，促使细胞因子或重组蛋白完全溶解。 有些蛋白，如重组人IL-13 Variant (产品编号：200-13A)虽然难溶，但却不需用摇床来加速溶解。其说明书上的备注为：Allow the reconstituted vial to sit at 4oC for at > 2 hours before use. (将重悬液在4oC静置2小时以上) 3. This solution
【求助】请教非单一且高度同源基因的RNAi问题，研究过小鼠骨钙素的请进
简繁 小鼠有三个串联的骨钙素（BGP，或OC）基因OG1、OG2、ORG，其中OG1和OG2在骨中表达，其基因序列高度同源但不完全一致，产物mRNA及蛋白亦稍有序列差别。 但是看文献中的骨钙素mRNA 的RT-PCR检测，均不提引物根据哪个基因设计；蛋白水平的检测亦不区分BGP1和BGP2，市面的小鼠骨钙素放免或酶免试剂盒亦笼统一用。 请教：我要做小鼠源成骨细胞的BGP RNAi，该以哪个基因为靶基因？随便选其中一个还是两个都照顾到？做RT
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