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文献和实验cyanine dye (Cy5―NHS; Amersham Pharmacia Biotech). These reagents react with primary amino groups in the antigen molecule, and may impair the antigen’s ability to react with the antibody after labeling. Phosphorylation of recombinant antibodies
You will need for 1° cDNA synthesis: 5 x SuperScript RTase buffer (Gibco-BRL) 5mM methyl dNTPs (Pharmacia, substitute 5m dCTP for dCTP) 100mM DTT (Gibco-BRL) oligo dT primer/adaptor [a-32P] dATP (~400Ci/mmol, Amersham or DuPont) Ribonuclease inhibitor
2-D Polyacrylamide Gel Electrophoresis
section into xylenes for 1 min. 3.Repeat steps 2-10,directly above,as for a paraffin-embed allowfullscreen='true'ded section on a slide. B.Reswelling 1.Remove Immobiline Drystrips (Amersham Pharmacia Biotech)from -20℃ and allow to equilibrate at RT
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