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文献和实验Northern protocol(RULES FOR RNA WORK)
1. Wear gloves at all time including filling pipet tip in racks, filling jars with Eppendorf tubes, and weighing chemicals to prepare solutions. 2. Pull weighing paper or weighing boat from middle of pile. Tap chemicals
in 20 ml of solvent mixture). After dispersion, the whole mixture is agitated during 15-20 min in an orbital shaker at room temperature.The homogenate is either filtrated (funnel with a folded filter paper) or centrifuged to recover the liquid phase
Alkaline Southern Blotting Procedure
plate that is wide enough to span the width of the tray over top. 12.Cut Zeta Probe GT membrane to size of gel excluding wells. Cut a "wick" out of 3mm paper. This should be the same width as your gel and long enough to drape over the glass plate
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