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文献和实验dT cellulose into a 2 ml Sarstedt screw cap tubes.2. Make fresh buffers: Note: Amounts are for 2 preps; scale up as necessary.♦ 2X NETS may need to be heated to go into solution.♦ 1X ETS should be heated to 65 ℃ for use.Protocol1. Resuspend
Acetone precipitation of protein
volume of acetone to -20°C. 2. Place protein sample in acetone-compatible tube, such as polypropylene and able to hold six times the sample. Screw cap tubes may help minimize sample losses. 3. Add six times the sample volume of cold (-20°C) acetone
Preabsorbing Antisera with C. elegans Acetone Powder
the equivalent of 0.1 g dry powder into each of several screw cap 1.5 ml eppendorf tubes. Microfuge for 10 minutes, discard the supernatant, and store the tubes frozen. 0.1 g of powder should be about 300 µl of packed wet material. 11. To preabsorb antisera
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