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文献和实验Kingfisher Flex 96 Plant DNA High Pure Protocol
to avoid those particles during the transfer in next step. 5. Carefully transfer 200ul supernatant to a 96-well Kingfisher Deep Well Plate, making sure not to disturb the pellet or transfer any debris. 6. Add 20ul/well of MagSi® Particles
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) or QiaFilter™ (Qiagen Inc.) plate positioned over a 96 deep-well collection block inside a vacuum manifold. Transfer 600 ul at a time, collecting the top half (with flocculent ppt.) first. Vacuum filter using approximately 300-500 mm Hg until filtration
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
over a piece of black cardboard, using a P-200 Gilson pick colony bringing with it the smallest possible volume of PBS. Pick 6 colonies in succession into a 96 well plate containing 20ul TRYPSIN/EDTA. Check using microscope that there are single colonies
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