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文献和实验Filter Binding Assay for EBNA-1
, 3ml syringePre-incubate filter with 100ul Binding Buffer at filter binding temperature (4C) until ready to bind DNA/EBNA-1 solution (e.g. conduct in 7th floor cold room).Incubate DNA fragment of interest with DNA with no known binding sites in a total
Filter Binding Assay for EBNA-1
temperature by addition of ice cold Binding Buffer to a final volume of 1000ul, incubation on ice until loading on filter Pass pre-incubation through filter by increased pressure supplied by syringe above filter. Remove syringe from filter apparatus. Load
Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper
by brief centrifugation. 6. Assemble an HiBind DNA column in a 2 ml collection tube (provided). Add 100ul of Equitation Buffer to each column. Wait 3-4 minutes at room temperature. Centrifuge at maximum speed for 30 seconds. 7. Carefully apply
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