100-1000ul Filter Tips 100-100

Filter Binding Assay for EBNA-1

, 3ml syringePre-incubate filter with 100ul Binding Buffer at filter binding temperature (4C) until ready to bind DNA/EBNA-1 solution (e.g. conduct in 7th floor cold room).Incubate DNA fragment of interest with DNA with no known binding sites in a total

Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper

by brief centrifugation. 6. Assemble an HiBind DNA column in a 2 ml collection tube (provided). Add 100ul of Equitation Buffer to each column. Wait 3-4 minutes at room temperature. Centrifuge at maximum speed for 30 seconds. 7. Carefully apply

支原体污染的特点及检验（2）

微量离心管　　无菌2ul, 20ul, 200ul, 1000ul Tips/ Pipetmans 方法： 此PCR反应是一种nested PCR，包括2阶段PCR反应，1st stage PCR结束后，取其反应物作2nd stage PCR，然后取2nd PCR产物作agarose gel electrophoresis，依DNA band之有无及片段大小来分析结果。 结果：使用UV light观察，并照相记录。 方法： 此PCR反应是一种nested PCR，包括2阶段PCR反应，1st