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- Biolegend（Covance）
- 中国/美国
- SIG-31126-kit
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
20℃
- 保质期：
12个月
- 库存：
大量
- 供应商：
康朗生物
- 规格：
kit
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文献和实验Blocking of unwanted non-specific staining
Blocking endogenous biotin Can be done with commercially available kits or by buying the isolated components of the kits, free biotin and free avidin. Can also be done with simpler reagents as below. 1- Wash twice in TBS 0.05M pH
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. x. Place 50 µL of the biotin-avidin-HRP mix on the preparation and cover with a coverslip. Incubate the slides for 40 min at room temperature in a humid chamber. xi. Wash slides in wash solution 1 for 15 min
- and streptavidin-conjugated markers to endogenous biotin and other non-specific interactions with neural tissue were overcome by several methods including the use of blocking kits, prolonged post-fixation of the tissue in paraformaldehyde, or omission of Triton X
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