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【简单介绍】
碱基修饰的脱氧核糖核酸(Base Modified dNTP)【详细说明】
TriLink 碱基修饰 DNA核苷修饰
许多化学物质都能以不同的方式修饰DNA的碱基，从而改变其配对性质而引起突变，改变碱基结构的化学修饰剂包括氨剂、羟化剂、烷化剂。
|
N-2002-1 |
2-Amino-6-Cl-purine-2'-deoxyriboside-TP |
1umole |
1657.5 |
|
N-2002-5 |
2-Amino-6-Cl-purine-2'-deoxyriboside-TP |
5umoles |
7905 |
|
N-2002-10 |
2-Amino-6-Cl-purine-2'-deoxyriboside-TP |
10umoles |
14450 |
|
N-2003-1 |
2-Amino-2'-deoxyadenosine-5'-TP |
1umole |
1317.5 |
|
N-2003-5 |
2-Amino-2'-deoxyadenosine-5'-TP |
5umoles |
6205 |
|
N-2003-10 |
2-Amino-2'-deoxyadenosine-5'-TP |
10umoles |
10625 |
|
N-2004-1 |
2-Aminopurine-2'-deoxyriboside-TP |
1umole |
1657.5 |
|
N-2004-5 |
2-Aminopurine-2'-deoxyriboside-TP |
5umoles |
7905 |
|
N-2004-10 |
2-Aminopurine-2'-deoxyriboside-TP |
10umoles |
14450 |
|
N-2006-1 |
5-Bromo-2'-deoxycytidine-5'-TP |
1umole |
1317.5 |
|
N-2006-5 |
5-Bromo-2'-deoxycytidine-5'-TP |
5umoles |
6205 |
|
N-2006-10 |
5-Bromo-2'-deoxycytidine-5'-TP |
10umoles |
10625 |
|
N-2008-1 |
5-Bromo-2'-deoxyuridine-5'-TP |
1umole |
467.5 |
|
N-2008-5 |
5-Bromo-2'-deoxyuridine-5'-TP |
5umoles |
1955 |
|
N-2008-10 |
5-Bromo-2'-deoxyuridine-5'-TP |
10umoles |
3485 |
|
N-2009-1 |
6-Chloropurine-2'-deoxyriboside-5'-TP |
1umole |
1317.5 |
|
N-2009-5 |
6-Chloropurine-2'-deoxyriboside-5'-TP |
5umoles |
6205 |
|
N-2009-10 |
6-Chloropurine-2'-deoxyriboside-5'-TP |
10umoles |
10625 |
|
N-2010-1 |
7-Deaza-2'-deoxyadenosine-5'-TP |
1umole |
892.5 |
|
N-2010-10 |
7-Deaza-2'-deoxyadenosine-5'-TP |
10umoles |
7055 |
|
N-2010-5 |
7-Deaza-2'-deoxyadenosine-5'-TP |
5umoles |
4335 |
|
N-2011-1 |
7-Deaza-2'-deoxyguanosine-5'-TP |
1umole |
1020 |
|
N-2011-10 |
7-Deaza-2'-deoxyguanosine-5'-TP |
10umoles |
8075 |
|
N-2011-5 |
7-Deaza-2'-deoxyguanosine-5'-TP |
5umoles |
4845 |
|
N-2012-1 |
2'-Deoxyinosine-5'-TP |
1umole |
1317.5 |
|
N-2012-10 |
2'-Deoxyinosine-5'-TP |
10umoles |
10625 |
|
N-2012-5 |
2'-Deoxyinosine-5'-TP |
5umoles |
6205 |
|
N-2016-1 |
5-Propynyl-2'-deoxycytidine-5'-TP |
1umole |
1317.5 |
|
N-2016-10 |
5-Propynyl-2'-deoxycytidine-5'-TP |
10umoles |
10625 |
|
N-2016-5 |
5-Propynyl-2'-deoxycytidine-5'-TP |
5umoles |
6205 |
|
N-2017-1 |
5-Propynyl-2'-deoxyuridine-5'-TP |
1umole |
1317.5 |
|
N-2017-10 |
5-Propynyl-2'-deoxyuridine-5'-TP |
10umoles |
10625 |
|
N-2017-5 |
5-Propynyl-2'-deoxyuridine-5'-TP |
5umoles |
6205 |
|
N-2019-1 |
2'-Deoxypseudouridine-5'-TP |
1umole |
|
|
N-2019-5 |
2'-Deoxypseudouridine-5'-TP |
5umoles |
|
|
N-2019-10 |
2'-Deoxypseudouridine-5'-TP |
10umoles |
|
|
N-2020-1 |
2'-Deoxyuridine-5'-TP |
1umole |
467.5 |
|
N-2020-10 |
2'-Deoxyuridine-5'-TP |
10umoles |
3485 |
|
N-2020-5 |
2'-Deoxyuridine-5'-TP |
5umoles |
1955 |
|
N-2022-1 |
5-Fluoro-2'-deoxyuridine-5'-TP |
1umole |
892.5 |
|
N-2022-10 |
5-Fluoro-2'-deoxyuridine-5'-TP |
10umoles |
7055 |
|
N-2022-5 |
5-Fluoro-2'-deoxyuridine-5'-TP |
5umoles |
4335 |
|
N-2023-1 |
5-Iodo-2'-deoxycytidine-5'-TP |
1umole |
1657.5 |
|
N-2023-10 |
5-Iodo-2'-deoxycytidine-5'-TP |
10umoles |
14450 |
|
N-2023-5 |
5-Iodo-2'-deoxycytidine-5'-TP |
5umoles |
7905 |
|
N-2024-1 |
5-Iodo-2'-deoxyuridine-5'-TP |
1umole |
892.5 |
|
N-2024-10 |
5-Iodo-2'-deoxyuridine-5'-TP |
10umoles |
7055 |
|
N-2024-5 |
5-Iodo-2'-deoxyuridine-5'-TP |
5umoles |
4335 |
|
N-2025-1 |
N6-Methyl-2'-deoxyadenosine-5'-TP |
1umole |
1657.5 |
|
N-2025-10 |
N6-Methyl-2'-deoxyadenosine-5'-TP |
10umoles |
14450 |
|
N-2025-5 |
N6-Methyl-2'-deoxyadenosine-5'-TP |
5umoles |
7905 |
|
N-2026-1 |
5-Methyl-2'-deoxycytidine-5'-TP |
1umole |
892.5 |
|
N-2026-10 |
5-Methyl-2'-deoxycytidine-5'-TP |
10umoles |
7055 |
|
N-2026-5 |
5-Methyl-2'-deoxycytidine-5'-TP |
5umoles |
4335 |
|
N-2027-1 |
O6-Methyl-2'-deoxyguanosine-5'-TP |
1umole |
2635 |
|
N-2027-10 |
O6-Methyl-2'-deoxyguanosine-5'-TP |
10umoles |
20995 |
|
N-2027-5 |
O6-Methyl-2'-deoxyguanosine-5'-TP |
5umoles |
11815 |
|
N-2028-1 |
N2-Methyl-2'-deoxyguanosine-5' |
1umole |
4250 |
|
N-2028-10 |
N2-Methyl-2'-deoxyguanosine-5' |
10umoles |
38250 |
|
N-2028-5 |
N2-Methyl-2'-deoxyguanosine-5' |
5umoles |
19550 |
|
N-2031-1 |
5-Nitro-1-indolyl-2'-deoxyribose-5'-TP |
1umole |
4250 |
|
N-2031-10 |
5-Nitro-1-indolyl-2'-deoxyribose-5'-TP |
10umoles |
38250 |
|
N-2031-5 |
5-Nitro-1-indolyl-2'-deoxyribose-5'-TP |
5umoles |
19550 |
|
N-2033-1 |
8-Oxo-2'-deoxyadenosine-5'-TP |
1umole |
1317.5 |
|
N-2033-10 |
8-Oxo-2'-deoxyadenosine-5'-TP |
10umoles |
10625 |
|
N-2033-5 |
8-Oxo-2'-deoxyadenosine-5'-TP |
5umoles |
6205 |
|
N-2034-1 |
8-Oxo-2'-deoxyguanosine-5'-TP |
1umole |
1317.5 |
|
N-2034-10 |
8-Oxo-2'-deoxyguanosine-5'-TP |
10umoles |
10625 |
|
N-2034-5 |
8-Oxo-2'-deoxyguanosine-5'-TP |
5umoles |
6205 |
|
N-2035-1 |
2-Thiothymidine-5'-TP |
1umole |
1657.5 |
|
N-2035-10 |
2-Thiothymidine-5'-TP |
10umoles |
14450 |
|
N-2035-5 |
2-Thiothymidine-5'-TP |
5umoles |
7905 |
|
N-2037-1 |
2'-Deoxy-P-nucleoside-5'-TP |
1umole |
1657.5 |
|
N-2037-10 |
2'-Deoxy-P-nucleoside-5'-TP |
10umoles |
14450 |
|
N-2037-5 |
2'-Deoxy-P-nucleoside-5'-TP |
5umoles |
7905 |
|
N-2038-1 |
5-Hydroxy-2'-deoxycytidine-5'-TP |
1umole |
1317.5 |
|
N-2038-10 |
5-Hydroxy-2'-deoxycytidine-5'-TP |
10umoles |
10625 |
|
N-2038-5 |
5-Hydroxy-2'-deoxycytidine-5'-TP |
5umoles |
6205 |
|
N-2039-1 |
5-Hydroxy-2'-deoxyuridine-5'-TP |
1umole |
|
|
N-2039-5 |
5-Hydroxy-2'-deoxyuridine-5'-TP |
5umoles |
|
|
N-2039-10 |
5-Hydroxy-2'-deoxyuridine-5'-TP |
10umoles |
|
|
N-2041-1 |
4-Thiothymidine-5'-TP |
1umole |
1317.5 |
|
N-2041-10 |
4-Thiothymidine-5'-TP |
10umoles |
10625 |
|
N-2041-5 |
4-Thiothymidine-5'-TP |
5umoles |
6205 |
|
N-2042-1 |
2-Thio-2'-deoxycytidine-5'-TP |
1umole |
1657.5 |
|
N-2042-10 |
2-Thio-2'-deoxycytidine-5'-TP |
10umoles |
14450 |
|
N-2042-5 |
2-Thio-2'-deoxycytidine-5'-TP |
5umoles |
7905 |
|
N-2043-1 |
6-Aza-2'-deoxyuridine-5'-TP |
1umole |
1317.5 |
|
N-2043-10 |
6-Aza-2'-deoxyuridine-5'-TP |
10umoles |
10625 |
|
N-2043-5 |
6-Aza-2'-deoxyuridine-5'-TP |
5umoles |
6205 |
|
N-2046-1 |
8-Chloro-2'-deoxyadenosine-5'-TP |
1umole |
1317.5 |
|
N-2046-10 |
8-Chloro-2'-deoxyadenosine-5'-TP |
10umoles |
10625 |
|
N-2046-5 |
8-Chloro-2'-deoxyadenosine-5'-TP |
5umoles |
6205 |
|
N-2048-1 |
5-Aminoallyl-2'-deoxycytidine-5'-TP |
1umole |
1317.5 |
|
N-2048-10 |
5-Aminoallyl-2'-deoxycytidine-5'-TP |
10umoles |
10625 |
|
N-2048-5 |
5-Aminoallyl-2'-deoxycytidine-5'-TP |
5umoles |
6205 |
|
N-2049-1 |
5-Aminoallyl-2'-deoxyuridine-5'-TP |
1umole |
892.5 |
|
N-2049-10 |
5-Aminoallyl-2'-deoxyuridine-5'-TP |
10umoles |
7055 |
|
N-2049-5 |
5-Aminoallyl-2'-deoxyuridine-5'-TP |
5umoles |
4335 |
|
N-2057-1 |
N4-Methyl-2'-deoxycytidine-5'-TP |
1umole |
1317.5 |
|
N-2057-10 |
N4-Methyl-2'-deoxycytidine-5'-TP |
10umoles |
10625 |
|
N-2057-5 |
N4-Methyl-2'-deoxycytidine-5'-TP |
5umoles |
6205 |
|
N-2058-1 |
2'-Deoxyzebularine-5'-TP |
1umole |
4250 |
|
N-2058-10 |
2'-Deoxyzebularine-5'-TP |
10umoles |
38250 |
|
N-2058-5 |
2'-Deoxyzebularine-5'-TP |
5umoles |
19550 |
|
N-5001-050 |
Biotin-16-Aminoallyl-2'-dUTP |
50nmoles |
3357.5 |
|
N-5001-1 |
Biotin-16-Aminoallyl-2'-dUTP |
1umole |
8925 |
|
N-5002-050 |
Biotin-16-Aminoallyl-2'-dCTP |
50nmoles |
3357.5 |
|
N-5002-1 |
Biotin-16-Aminoallyl-2'-dCTP |
1umole |
8925 |
|
N-5004-050 |
N4-Biotin-OBEA-2'-deoxycytidine-5'-TP |
50nmoles |
3357.5 |
|
N-5004-1 |
N4-Biotin-OBEA-2'-deoxycytidine-5'-TP |
1umole |
8925 |
|
N-5006-050 |
Biotin-16-7-Deaza-7-Aminoallyl-2'-dGTP |
50nmoles |
3655 |
|
N-5006-1 |
Biotin-16-7-Deaza-7-Aminoallyl-2'-dGTP |
1umole |
12325 |
|
N-5007-0100 |
Dabcyl-5-3-Aminoallyl-2'-dUTP |
100nmoles |
3357.5 |
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文献和实验Modified Eberwine (antisense) RNA Amplification Protocol
Modified Eberwine ("antisense") RNA Amplification Protocol Chris Barry, MD, PhD, Pat Brown Lab, 4/22/99 barry_c@cmgm.stanford.edu The optimal range for amplifying from mRNA is 20-100 nanograms. The optimal range for total RNA
带 可能原因： · 酶用量过多 · 模板纯度较低 · dNTP、镁离子浓度过高 · 循环次数过多 解决方法： · 减少用酶量或使用特异性高的热启动酶扩增 · 纯化模板 · 减少 dNTP 用量，摸索镁离子浓度 · 减少循环次数 问题 4：二聚体及非特异性产物 一般小于 100bp 的条带可基本判断为引物二聚体 主要原因： · 引物设计不合理，引物自身具有互补性 · 引物浓度不适 · 镁离子浓度过高，退火温度过低 解决方法： · 重新设计引物并进行 BLAST 检测 · 降低镁离子浓度，提高退火温度 ·
领域奠基之作：Cell 重磅报道可胞外展示的糖基化修饰 RNA——glycoRNA
多糖可以修饰脂质和蛋白质，调节所有生命领域的分子间和分子内的相互作用。因此，许多基本过程，如胚胎形成，宿主病原体识别和肿瘤免疫相互作用都是依赖于糖基化的。而通常人们认为，RNA 不是糖基化的主要目标。 RNA 作为另一种生物高聚物，是已知生命的中心。虽然 RNA 通常局限于 4 个碱基，但是转录后修饰（post-transcriptional modifications，PTMs）可以显著扩大 RNA 的多样性。目前已鉴定有超过 100 种 PTMs，但是除了少数基于单糖的 tRNA 修饰
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