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- 文献和实验
- 技术资料
- 浓度:
1mg/ml
- 应用范围:
科学研究
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Chicken,Dog,Cow,Rabbit,Sheep
- 标记物:
PE HRP Gold Cy5.5 Cy7 RBITC PE-CY5.5 PE-CY7 APC FITC Cy3 Cy1774
- 克隆性:
Polyclonal
- 保存条件:
-20℃
- 形态:
液态/冻干粉
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human/mouse/Rat
- 规格:
200 ul (20 Western Blots)
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文献和实验, the resulting acetylated peptides of interest can be quantitated using selected reaction monitoring (SRM)-MS with stable isotope dilution. Here, we describe the enrichment of lysine acetylated peptides from typsin digested mouse liver mitochondria
Identifying Acetylated Proteins in Mitosis
to search for acetylated proteins in mitosis (2). First, we synchronized cells in mitosis and used a polyclonal anti-acetyl-Lysine antiserum to immunoprecipitate acetylated proteins, followed by their identification by LC-ESI-MS/MS. We then confirmed
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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