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文献和实验Plasma Protein-Binding Methods in Drug Discovery
This chapter focuses on the three most widely used in vitro protein binding techniques in pharmaceutical research, which each reflect a diversity of speed, data quality, and complexity. Chromatographic separation using a human serum albumin
Free-Flow Electrophoresis System for Plasma Proteomic Applications
-, and albumin- fractions that were furthered for gel-based analysis. Under denaturing condition plasma proteins were separated into 96 fractions. Each fraction can be supplied for in-solution digestion and further LC-MS/MS analysis. From a single FFE fraction 46
Blood plasma is believed the most complex human-derived proteome, containing other tissue proteome subsets. Almost all body cells communicate with the plasma, either directly or through tissues or biological fluids, and many of these cells
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