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低温
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现货
- 供应商:
上海善然生物科技有限公司
Related Categories Biochemicals and Reagents, Biomaterials, Materials Science, Nucleosides, Nucleotides, Oligonucleotides, Oligonucleotide Polymers and Copolymers,
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Quality Level PREMIUM
storage temp. −20°C
Description
Preparation Note
Prepared from ADP with polynucleotide phosphorylase
Application
Polyadenylic acid (poly-A) is used to evaluate binding on cationic liposomes doped with non-ionic nucleolipids. Poly-A is used in small molecule mRNA targeted drug development to evaluate the binding of potential therapeutic agents such as the Isoquinoline group of alkaloids.
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文献和实验Analysis of Genomic DNA by Southern Hybridization
DNA at 65µC for overnight in a hybridization incubator (Robbins Scientific). Probe supression Prepare the supression mixture 10µl labelled BAC probe 40µl cold nonspecific DNA 50µl poly dG-dT/dA-dC (0.25mg/ml) (Pharmacia
In Situ HYBRIDIZATION TO TISSUE SECTIONS
as necessary but be sure the final pH is 7.5-8. Allow to cool to RT before using. 10X PBS 1.3M NaCl (58.44) = 74.0g NaCl 70mM Na2HPO4 (142.0) = 9.94g anhydrous 30mM NaH2PO4 (120.0) = 3.6g anhydrous Polylysine [SIGMA P1149 (poly-D) or SIGMA P
,将二者充分混合后,分装成小份,-20℃存放,用时再取出冻融,余者弃去。 (2)工作液(临用前配): 取储备液(1mg/ml)按1:40稀释,充分混合,即得约含Pro.K为25mg/ml的工作液。 (3)关于P-K缓冲液的配制: 称取上述试剂,充分混合即可。 ②1mol/L 的Tris-HCl(pH8.0): 先将Tris于800ml ddH2 O中溶解,用HCl将pH调至8.0,ddH
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