- 询价
- Cell Signaling Technology
- 9874
- USA
- 2025年11月21日
- W
- Rabbit
- H,M,R
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- 详细信息
- 询价记录
- 技术资料
- 抗体英文名：
Phospho-SREBP-1c (Ser372) Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Ser372 of human SREBP-1c protein
- 应用范围：
W
- 宿主：
Rabbit
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
H,M,R
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Transfected Only | 150 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-SREBP-1c (Ser372) Antibody recognizes transfected levels of SREBP-1c protein only when phosphorylated at Ser372. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser372 of human SREBP-1c protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from serum-starved 293T cells treated with resveratrol (50 μM, 1 hr), transfected with a GST-tagged cDNA expression construct encoding either full-length human wild-type SREBP-1c (GST-hSREBP-1c) or full-length mutant SREBP-1c (GST-hSREBP-1c (S372A)), using Phospho-SREBP-1c (Ser372) Antibody (upper) or GST (91G1) Rabbit mAb #2625 (lower). Both expression vectors were generated in Dr. Mengwei Zang's laboratory at Boston University School of Medicine. |
| Background | Sterol regulatory element–binding proteins (SREBPs) are basic helix-loop-helix–leucine zipper transcription factors (1,2). Inactive precursor forms of SREBPs are bound to endoplasmic reticulum (ER) membranes (1,2). When cells are starved for cholesterol, SREBPs move from the ER to the Golgi apparatus with the help of SREBP cleavage–activating protein (SCAP) (1,2). In the Golgi apparatus, precursor SREBPs are then cleaved by two proteases, Site-1 protease (S1P) and Site-2 protease (S2P) (1,2). The released N-terminal domains enter the nucleus and bind to sterol response elements in the promoters of a variety of genes responsible for the synthesis of cholesterol (1,2). SREBPs also activate the expression of genes involved in the synthesis of fatty acids and lipids (1,2). Among the isoforms of SREBPs, SREBP-1c activates all lipogenic genes in the liver (3). SREBP-1c has been implicated to contribute to the development of hepatic steatosis in the rodent model of insulin resistance and obesity (3). Recent studies have shown that AMPK interacts with and directly phosphorylates SREBP-1c and SREBP-2 (4). Phosphorylation of SREBP-1c at Ser372 by AMPK, which is stimulated by polyphenols and metformin, inhibits the proteolytic cleavage of SREBP-1c and therefore suppresses the expression of its target genes in the liver (4). This process leads to the reduction of lipid synthesis and accumulation in the liver (4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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