Phospho-Semaphorin 4B (Ser825)

5622:

Western Blotting

Phospho-Semaphorin 4B (Ser825) Antibody detects endogenous levels of semaphorin 4B protein only when phosphorylated at Ser825.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser825 of human semaphorin 4B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from MKN-45 cells, untreated or SU11274-treated (1 μM for 2 hr), using Phospho-Semaphorin 4B (Ser825) Antibody (upper) and Semaphorin 4B Antibody #3355 (lower).

The semaphorin family of proteins is involved in axon guidance, cell migration, angiogenesis, and immune response. Plexins and neuropilins bind with high affinity to semaphorins to mediate their functions. Semaphorins are divided into seven classes of secreted or membrane-bound proteins. Members of Class 4 semaphorins include 4A through 4G; semaphorin 4B is a membrane-associated protein (1,2). Semaphorin 4B binds to the CLCP1 receptor and regulates cell motility (3). Furthermore, semaphorin 4B, like many other semaphorins, has a PDZ domain-binding motif at the carboxy terminus that interacts with PSD95 and localizes to the post-synaptic membrane (4).

The phosphorylation site at Ser825 was identified at Cell Signaling Technology using PTMScan^® Technology, a proprietary proteomic method for post-translational modification site discovery (5).

1. Pasterkamp, R.J. and Kolodkin, A.L. (2003) Curr Opin Neurobiol 13, 79-89.
2. Kruger, R.P. et al. (2005) Nat Rev Mol Cell Biol 6, 789-800.
3. Nagai, H. et al. (2007) Oncogene 26, 4025-31.
4. Burkhardt, C. et al. (2005) FEBS Lett 579, 3821-8.
5. Moritz, A. et al. (2010) Sci Signal 3, ra64.

• Moritz, A. et al. (2010) Sci Signal 3, ra64. Applications: Western Blotting

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