- 询价
- Cell Signaling Technology
- 5443
- USA
- 2025年10月24日
- W, IP, IHC-P, F
- Rabbit
- H,M,R
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- 详细信息
- 技术资料
- 抗体英文名：
Ikaros Antibody
- 抗原：
synthetic peptide corresponding to residues surrounding Pro329 of human Ikaros protein
- 应用范围：
W, IP, IHC-P, F
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 适应物种：
H,M,R
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P F | H M R | Endogenous | 50-70 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Ikaros Antibody detects endogenous levels of total Ikaros protein. It is expected to recognize all isforms of Ikaros protein produced by differential splicing. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro329 of human Ikaros protein. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western analysis of extracts from various cell lines using Ikaros Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse spleen using Ikaros Antibody. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Ikaros Antibody. Note staining of leukocytes. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lymphoma using Ikaros Antibody. Flow Cytometry
Flow cytometric analysis of Jurkat cells using Ikaros Antibody (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (red). |
| Background | Ikaros family proteins, characterized by the presence of N-terminal zinc finger DNA-binding domain and C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily. Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus (1). They can form homodimers as well as heterdimers with other members of the Ikaros family. Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization (2).Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) and LYF-1 (Lymphoid transcription factor 1), is expressed abundantly in blood cells. Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor (3,4). Imbalanced expression of different Ikaros isoforms as well as deletions of Ikaros are associated with hematologic malignancies in humans (5-7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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