Renin Antibody

5250:

Western Blotting

Renin Antibody detects transfected levels of human Renin protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu308 of human renin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from COS-7 cells, mock transfected or transfected with a construct expression human Renin, using Renin Antibody.

Renin is a secreted proteinase whose enzymatic activity is to convert angiotensinogen into angiotensin I in the plasma, initiating a process that results in an elevation of blood pressure and increased sodium retention by the kidney (1). Renin is synthezed in kidney as a procursor, prorenin, which is released into circulation. Both renin and prorenin can bind to (pro)renin receptor and induce angiotensin-independent signaling events leading to activation of MAPKs and up-regulation of TGF-β1 and matrix proteins (2, 3). Defects in renin can cause renal tubular dysgenesis, a severe autosomal recessive disorder of renal tubular development (4, 5).

1. Castrop, H. et al. (2010) Physiol Rev 90, 607-73.
2. Ichihara, A. et al. J Nephrol 22, 306-11.
3. Nguyen, G. (2010) Ann Med 42, 13-8.
4. Lacoste, M. et al. (2006) J Am Soc Nephrol 17, 2253-63.
5. Gubler, M.C. and Antignac, C. (2010) Kidney Int 77, 400-6.

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• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.