Thymidylate Synthase Antibody

3766:

Western Blotting

Thymidylate Synthase Antibody detects endogenous levels of total thymidylate synthase protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human thymidylate synthase. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell types using Thymidylate Synthase Antibody.

The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides (1,2, reviewed in 3). This process is catalyzed by thymidylate synthase (TS or TYMS), a homodimer composed of two 30 kDa subunits. TS is an intracellular enzyme that provides the sole de novo source of thymidylate, making it a required enzyme in DNA biosynthesis with activity highest in proliferating cells (1). Being the exclusive source of dTMP, investigators have concluded that TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU) (1-5). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head, and neck. Research studies have dmonstrated that patients with metastases expressing lower levels of TS have a higher response rate to treatment with 5-FU than patients with tumors that have increased levels of TS (5). Researchers continue to investigate TS expression in different types of cancers (6-10).

1. Johnston, P.G. et al. (1991) Cancer Res 51, 6668-76.
2. Aschele, C. et al. (2002) Ann Oncol 13, 1882-92.
3. Jackman, A.L. and Calvert, A.H. (1995) Ann Oncol 6, 871-81.
4. Van Triest, B. et al. (2000) J Histochem Cytochem 48, 755-60.
5. Johnston, P.G. et al. (1994) J Clin Oncol 12, 2640-7.
6. Kwon, H.C. et al. (2007) Ann Oncol 18, 504-9.
7. Allegra, C.J. et al. (2002) J Clin Oncol 20, 1735-43.
8. Allegra, C.J. et al. (2003) J Clin Oncol 21, 241-50.
9. Tsourouflis, G. et al. (2008) Dig Dis Sci 53, 1289-96.
10. Kim, S.H. et al. (2009) Am J Clin Oncol 32, 38-43.
11. Chu, E. et al. (1993) Mol Pharmacol 43, 527-33.
12. Kim, H.P. et al. (2009) PLoS One 4, e5933.

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• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.