- 询价
- Cell Signaling Technology
- 3453
- USA
- 2025年11月24日
- W, IP, IF-F
- Rabbit
- H,M,R
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-Doublecortin (Ser334) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser334 of doublecortin
- 应用范围：
W, IP, IF-F
- 宿主：
Rabbit
- 保质期：
详见说明书
- 适应物种：
H,M,R
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-F | H M R | Endogenous | 45 | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Phospho-Doublecortin (Ser334) Antibody detects endogenous levels of doublecortin only when phosphorylated at Ser334. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser334 of doublecortin. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from neonatal mouse brain and fetal rat brain using Phospho-Doublecortin (Ser334) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho- or non-phosphopeptide and by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer. IF-F
Confocal immunofluorescent analysis of postnatal day 14 rat brain, untreated (left) or λ-phosphatase treated (right), using Phospho-Doublecortin (Ser334) Antibody (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Mutations in Doublecortin cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5 and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331 and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5). Doublecortin phosphorylated at Ser334 is enriched in growth cones and affects neurite outgrowth and neuronal migration (3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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