- 询价
- Cell Signaling Technology
- 3522
- USA
- 2025年12月05日
- W
- Rabbit
- H
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-PNK1 (Ser114/Thr118) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues around Ser114 and Thr118 of human PNK1
- 应用范围：
W
- 宿主：
Rabbit
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 59 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-PNK1 (Ser114/Thr118) Antibody detects endogenous levels of PNK1 only when phosphorylated on Ser114 and Thr118. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser114 and Thr118 of human PNK1. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts of 293 and HeLa cells, either untreated or treated with UV radiation, using Phospho-PNK1 (Ser114/Thr118) Antibody (upper). β-actin Antibody #4967 (lower) was used to control for loading. |
| Background | PNK (polynucleotide kinase) is a DNA repair enzyme that participates in single strand break repair and non-homologous end rejoining (NHEJ) for double strand breaks. PNK possesses a 5'-DNA kinase activity and a 3'-DNA phosphatase activity (1,2). It has three domains, a C-terminal kinase domain, a central phosphatase domain, and an N-terminal forkhead associated (FHA) domain that is responsible for protein-protein interactions. Reduction in expression of PNK by RNAi sensitizes cells to ionizing radiation and topoisomerase I inhibitors (3) Phosphorylation of PNK1 at Ser114/ Thr118 was independently identified at Cell Signaling Technology (CST) using PhosphoScan® , CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PNK1 at Ser114/ Thr118 was observed in extracts of various cell lines following UV treatment. For additional information please visit PhosphoSitePlus® , CST's modification site knowledgebase, at www.phosphosite.org. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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