PPIG Antibody

3803:

Western Blotting

PPIG Antibody detects endogenous levels of total PPIG protein. This antibody also recognizes a unidentified protein at 49 kDa.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu391 of human PPIG. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from various cell lines using PPIG Antibody.

PPIG belongs to a highly conserved class of cyclophilins that function as peptidyl-prolyl-isomerases (PPIases) to catalyze the conversion of cis-proline to trans-proline in a polypeptide chain (1-4). PPIG contains an amino-terminal cyclophilin domain followed by Nopp140 repeats that are involved in its function as a nuclear chaperone (5). The carboxy-terminal of PPIG contains a SR (arginine-serine dipeptide repeat) domain (3,4) that is involved in pre-mRNA splicing and processing (6). PPIG interacts with the carboxy-terminal domain of RNA polymerase II as well as several other SR family splicing factors. These interactions lead to changes in localization and conformation and suggest a regulatory role in transcription and pre-mRNA splicing in the elongating RNA polymerase complex (7,8). PPIG is found in the nuclear matrix and nuclear speckles and is involved in the regulation of gene expression. PPIG shows a predominantly diffuse cytoplasmic distribution at the onset of mitosis, and in late telophase the isomerase is recruited to the newly formed nuclei (9).

1. Fischer, G. et al. (1989) Nature 337, 476-8.
2. Freskgård, P.O. et al. (1992) Science 258, 466-8.
3. Nestel, F.P. et al. (1996) Gene 180, 151-5.
4. Mortillaro, M.J. and Berezney, R. (1998) J Biol Chem 273, 8183-92.
5. Meier, U.T. and Blobel, G. (1992) Cell 70, 127-38.
6. Zahler, A.M. et al. (1993) Science 260, 219-22.
7. Lin, C.L. et al. (2004) Biochem Biophys Res Commun 321, 638-47.
8. Bourquin, J.P. et al. (1997) Nucleic Acids Res 25, 2055-61.
9. Dubourg, B. et al. (2004) J Biol Chem 279, 22322-30.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.