Phospho-Tyrosine Hydroxylase (

3370:

Immunoprecipitation , Western Blotting

Phospho-Tyrosine Hydroxylase (Ser31) Antibody detects endogenous levels of tyrosine hydroxylase only when phosphorylated at Ser31.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Ser31 of mouse tyrosine hydroxylase. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or λ phosphatase-treated, using Phospho-Tyrosine Hydroxylase (Ser31) Antibody (upper) and Tyrosine Hydroxylase Antibody #2792 (lower).

Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9).

1. Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
2. Bodeau-Péan, S. et al. (1999) J Biol Chem 274, 3469-75.
3. Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43.
4. Lew, J.Y. et al. (1999) Mol Pharmacol 55, 202-9.
5. Vié, A. et al. (1999) J Biol Chem 274, 16788-95.
6. Lindgren, N. et al. (2000) J Neurochem 74, 2470-7.
7. Moy, L.Y. and Tsai, L.H. (2004) J Biol Chem 279, 54487-93.
8. Lehmann, I.T. et al. (2006) J Biol Chem 281, 17644-51.
9. Saraf, A. et al. (2007) J Biol Chem 282, 573-80.

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• 2791 Phospho-Tyrosine Hydroxylase (Ser40) Antibody
• 2792 Tyrosine Hydroxylase Antibody
• 2506 CDK5 Antibody
• 2680 p35/25 (C64B10) Rabbit mAb
• 2306 DARPP-32 (19A3) Rabbit mAb
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 9998 BSA
• 9999 Nonfat Dry Milk

For Research Use Only. Not For Use In Diagnostic Procedures.