- 询价
- Cell Signaling Technology
- 2442
- USA
- 2025年12月02日
- W, IF-IC, F
- Rabbit
- M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
HSP27 Antibody (Rodent Preferred)
- 抗原：
synthetic peptide corresponding to rat HSP27
- 应用范围：
W, IF-IC, F
- 宿主：
Rabbit
- 供应商：
CST
- 适应物种：
M,R
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | M R | Endogenous | 27 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | HSP27 Antibody (Rodent Preferred) detects endogenous levels of total HSP27 protein. The antibody does not cross-react with other heat shock proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to rat HSP27. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from C2C12, PC12, HeLa and COS cells using HSP27 Antibody (Rodent Preferred). Flow Cytometry
Flow cytometric analysis of C2C12 cells using HSP27 Antibody (Rodent Preferred) (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of C2C12 cells using HSP27 Antibody (Rodent Preferred) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验IMMUNOSCREENING OF WITH ANTIBODY PROBES
in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody
Immunohistochemical Localization of TNFα and Its Receptors in the Rodent Central Nervous System
followed by fixation and cryostat sectioning in 30–40 μm slices. Free-floating sections are then incubated with the primary antibody followed by incubation with a secondary antibody species-specific for the type of animal in which the primary antibody
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人热休克蛋白27 ( HSP27 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 HSP27 单抗包被于酶标板上，标准品和样品中的 HSP27与单抗结合，加入生物素化的抗人 HSP27 ，形成免疫复合物连接在板上，辣根
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