- 询价
- Cell Signaling Technology
- 2765
- USA
- 2025年10月26日
- W, IP
- Rabbit
- H,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Ribosomal Protein L13a Antibody
- 抗原：
synthetic peptide corresponding to the sequence of human ribosomal protein L13a
- 应用范围：
W, IP
- 宿主：
Rabbit
- 适应物种：
H,Mk
- 供应商：
CST
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H Mk | Endogenous | 23 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Ribosomal Protein L13a Antibody detects endogenous levels of total ribosomal protein L13a. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ribosomal protein L13a. Antibodies are purified by peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various cell types using Ribosomal Protein L13a Antibody. |
| Background | Ribosomal protein L13a (RPL13a, 60S ribosomal protein L13a) is a member of the L13 ribosomal protein family and a structural component of the 60S ribosomal subunit (1). RPL13a appears to play an important role in transcript-specific translational silencing. Interferon-γ induces the phosphorylation of RPL13a and triggers the release of this protein from the 60S ribosomal subunit (2). Free RPL13a protein binds to the GAIT (interferon-γ-activated inhibitor of translation) complex at the 3'-UTR of ceruloplasmin (Cp) mRNA to repress Cp expression (2). RPL13a bound to the GAIT complex interacts with eIF4G, which prevents the recruitment of 43S ribosomal subunit and results in transcript-specific translation suppression (3). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L
Today, monoclonal antibodies (mAbs) form the largest category of biopharmaceuticals in clinical trials and their number is expanding rapidly (DataMonitor 2007). The antibodies or functional antibody fragments are being produced in artificial
Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
(five or six His residues) placed at either the C- or N-terminus of a recombinant protein can form a stable chelate with immobilized transition metals. This allows fractionation of the target protein to 90–95% purity levels in a single chromatographic step (2 –4 ). Metals
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
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