- 询价
- Cell Signaling Technology
- 2543
- USA
- 2025年12月12日
- W
- Rabbit
- H,Hm,Mk,M,R,Dm,X,B
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-cdc2 (Thr14) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Thr14 of human cdc2
- 应用范围：
W
- 宿主：
Rabbit
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 级别：
详见MSDS文件
- 适应物种：
H,Hm,Mk,M,R,Dm,X,B
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Dm=D. melanogaster X=Xenopus B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H Hm Mk (M) (R) (Dm) (X) (B) | Endogenous | 34 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-cdc2 (Thr14) Antibody detects endogenous levels of cdc2 (CDK1) only when phosphorylated at Thr14. Based on sequence similarity, the antibody may cross-react with CDK2 and CDK3. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr14 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from hydroxyurea-treated HT29 cells, aphidicolin-treated HeLa cells, and asynchronous and mitotic CHO cells using Phospho-cdc2 (Thr14) Antibody. Western Blotting
Western blot analysis of extracts from asynchronous and mitotic HeLa cells using Phospho-cdc2 (Thr14) Antibody. |
| Background | The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins. A polyclonal antibody was raised against purified ST-IV. A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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