ARP2 Antibody

3128:

Western Blotting

ARP2 Antibody detects endogenous levels of total ARP2 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human ARP2.

Western Blotting

Western blot analysis of extracts from various cell types using ARP2 Antibody.

Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3).

1. Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726.
2. Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463.
3. Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.

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• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.