- 询价
- Cell Signaling Technology
- 9509
- USA
- 2025年10月29日
- W, IF-IC
- Rabbit
- M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific)
- 抗原：
synthetic peptide corresponding to residues surrounding Asp353 of mouse caspase-9
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 保质期：
详见说明书
- 适应物种：
M
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | M | Endogenous | 37 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) detects endogenous levels of the 37kDa subunit of mouse caspase-9 only after after cleavage at aspartic acid 353. It does not cross-react with full length caspase-9 or with other caspases at endogenous levels. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp353 of mouse caspase-9. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from L929 cells, untreated or staurosporine-treated, using Cleaved Caspase-9 (Asp353) Antibody (upper) or Caspase-9 Antibody #9504 (lower). IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells, staurosporine-treated (left) or untreated (right), using Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
Assessment of Insulin Secretion in the Mouse
from the cleavage site ( 4 ). In the rat, two separate 110-amino acid preproinsulins are transcribed from two nonallelic preproinsulin genes, from which two forms of insulin and C-peptide are subsequently cleaved ( 1 ) ( see Fig
或 C6 cells 作为 cleaved caspase-3 的阳性参照。更多靶点的阳性对照，请登陆CST网站——研究——靶标的阳性对照处理。图二 蛋白质印迹分析（图源自CST Caspase-3 Antibody #9662 抗体说明书）为什么所有的实验都要设置对照？无论是初高中刚接触生物学实验设计还是上研究生后发表 SCI 文章，老师们以及 reviewer 都会敲着「黑板」强调强调再强调，每一个实验都必须有对照！因为设置适当的阴性、阳性甚至空白对照对于实验结果的准确、有效阐释至关重要！阅历丰富
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