Phospho-CDK2 (Thr160) Antibody

2561:

ELISA Peptide , Flow , Immunoprecipitation , Western Blotting

Phospho-CDK2 (Thr160) Antibody detects endogenous levels of CDK2 only when phosphorylated at threonine 160. The antibody weakly cross-reacts with cdc2 phosphorylated at Thr161. It does not cross-react with other phosphorylated cyclin dependent kinases.

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr160 of human CDK2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa cells synchronized in G0, G1/S or Mitosis, using Phospho-CDK2 (Thr160) Antibody.

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-CDK2 (Thr160) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-CDK2 (Thr160)-positive cells.

Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).

1. Morgan, D.O. (1995) Nature 374, 131-134.
2. Poon, R.Y. et al. (1996) J. Biol. Chem. 271, 13283-13291.
3. Gu, Y. et al. (1992) EMBO J. 11, 3995-4005.
4. Fesquet, D. et al. (1993) EMBO J. 12, 3111-3121.
5. Morgan, D.O. (1997) Annu. Rev. Cell Dev. Biol. 13, 261-291.

• Chiariello, M. et al. (2000) Regulation of cyclin-dependent kinase (CDK) 2 Thr-160 phosphorylation and activity by mitogen-activated protein kinase in late G1 phase. Biochem. J. 349, 869-876. Applications: Western Blot
• Lents, N. H. et al. (2002) Stimulation of the Raf/MEK/ERK cascade is necessary and sufficient for activation and Thr-160 phosphorylation of a nuclear-targeted CDK2. J. Biol. Chem. 277, 47469-47475. Applications: Western Blotting

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For Research Use Only. Not For Use In Diagnostic Procedures.