- 询价
- Cell Signaling Technology
- 5631
- USA
- 2025年11月07日
- F
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Met (11C4) Mouse mAb (Flow Specific)
- 抗原：
human cancer cell lines
- 应用范围：
F
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
H
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
200 ul (100 tests)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 200 ul (100 tests) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| F | H | Endogenous | 145 | Mouse IgG1 |
| Protocols |
* Product-specific protocol. |
|---|---|
| Specificity / Sensitivity | Met (11C4) Mouse mAb (Flow Specific) detects endogenous levels of total Met protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with human cancer cell lines. Flow Cytometry
Flow cytometric analysis of live Ramos cells (red) and live MKN-45 cells (blue) using Met (11C4) Mouse mAb (Flow Specific). |
| Background | Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45 kDa α- and 145 kDa β-subunits (1,2). The α-subunit and the amino-terminal region of the β-subunit form the extracellular domain. The remainder of the β-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3). These fundamental events are important for all of the biological functions involving Met kinase activity. The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4). Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation. Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5). Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast. Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验T-Cell Activation Using mAb to CD3
Introduction Mature T cells recognize and respond to the antigen/MHC complex through their antigen-specific receptors (TCR). The most immediate consequence of TCR activation is the initiation of signaling pathways including induction
Intracellular Immunofluorescent Staining for Flow Cytometry
on a flow cytometer. Foxp3 Staining Protocol The following protocol is for intracellular staining of Human Foxp3 (clone PCH101, cat. 4776 ). Please refer to specific clones for the corresponding protocol. We have also determined that this kit
Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice
the expression of mouse heavy or light chains, the endogenous mouse Ig (mIg) loci must be silenced by gene-targeting techniques. Subsequently, to obtain antigen-specific mAbs, conventional immunization protocols can be followed and the mAb technique used (fusion
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