- 询价
- Cell Signaling Technology
- 2625
- USA
- 2025年08月30日
- W, IP, IF-IC
- All
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
GST (91G1) Rabbit mAb
- 抗原：
GST fusion protein
- 应用范围：
W, IP, IF-IC
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
All
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IP IF-IC | All | Transfected Only | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | GST (91G1) Rabbit mAb detects transfected GST fusion proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a GST fusion protein. Western Blotting
Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing GST-Bad (+), using GST (91G1) Rabbit mAb (left) and Bad Antibody #9292 (right). IF-IC
Immunocytochemical staining of cells expressing GST-Bad protein (left) or cells without transfected protein (right), using GST (91G1) Rabbit mAb. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验GST-fusion protein injection into rabbits
Boost: For second injection (a month later 5-20 mg of fusion protein is required/boost injection). Two rabbits are being used to generate antibody to the same antigen. One rabbit is injected with the native form of GST-fusion protein, and the second
Affinity Purification of Proteins Binding to GST Fusion Proteins
the use of proteins fused to glutathione?S ?transferase (GST fusion proteins) to affinity purify other proteins, a technique also known as GST pulldown purification. The describes a strategy in which a GST fusion protein is bound to agarose affinity beads
优化的GlycoWorks HILIC SPE流程用于mAb型样品中N-糖的定量与稳定回收
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