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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH conjugated synthetic peptide derived from hu MEK6
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 保存条件:
2-8℃短期保存或<-20℃长期保存1年
- 克隆性:
多克隆
- 适应物种:
hu, mo, rat
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
- 浓度:
1mg/1ml
- 抗体名:
TBP抗体 TATA结合蛋白TBP抗体
- 规格:
0.2ml/0.1ml/200μg
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文献和实验Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
Using FRET to Monitor Protein-Induced DNA Bending: The TBP-TATA Complex as a Model System
Proteins that bind to DNA can elicit changes in DNA conformation, such as bending and looping, which are important signals for later events such as transcription. TATA-binding protein (TBP) is one example of a protein that elicits
Western Blot of TBP from TBP-GST bacteria
Culture bacteria 1. Grow glycerol stock A13 (TBP-GST in BL21) in 10ml LB and 10μl ampicillin (1:1000) 2. Shake @ 37℃ ON Induce bacteria 3. Adjust culture with LB until ON culture OD [@ 600nm is ~0.6-0.8 4. Induce with 40mM IPTG (made fresh
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