- 询价
- R&D
- 美国/中国
- xy-639R
- 2025年07月07日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Neuronal Nicotinic Acetylcholine Receptor, β2-Subunits, Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal Antibody against Neuronal Nicotinic Acetylcholine Receptor, β2-Subunits Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone 270 Form Ascites Host Rat Species Reactivity Neuronal Nicotinic Acetylcholine Receptor, β2-Subunits, Monoclonal AntibodyRodent, Chicken IsoType IgG2a [Ab] 5-6 mg/mL Specificity This antibody was generated in rats against purified neuronal nicotinic acetylcholine receptors (AChRs) from chicken brain. It binds to the extracellular surface of neuronal AChR β2-subunits. The antibody binds to native subunits as well as denatured subunits although with lower affinity. Clone 270 is useful for immunoisolating and pharmacologically characterizing AChRs, immunopurification of AChRs and is also useful histologically. The antibody cross-reacts with chicken and rodent AChRs as well as human, albeit with lower affinity. Uses This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP). Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:1,000*
- IF: 1:500
- IP: 1:150
Notes Neuronal Nicotinic Acetylcholine Receptor, β2-Subunits, Monoclonal AntibodyPredicted MW = 57 kDa Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Whiting PJ, Liu R, Morley BJ, Lindstrom JM. Structurally different neuronal nicotinic acetylcholine receptor subtypes purified and characterized using monoclonal antibodies. J Neurosci 7:4005-4016, 1987.
Whiting PJ, Schoepfer R, Swanson LW, Simmons DM, Lindstrom JM. Functional acetylcholine receptor in PC12 cells reacts with a monoclonal antibody to brain nicotinic receptors. Nature 327:515-518, 1987.
Swanson LW, Simmons DM, Whiting PJ, Lindstrom JM. Immunohistochemical localization of neuronal nicotinic receptors in the rodent central nervous system. J Neurosci 7:3334-3342, 1987.
Whiting PJ, Lindstrom JM. Pharmacological properties of immuno-isolated neuronal nicotinic receptors. J Neurosci 6:3061-3069, 1986.
Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Neuronal Nicotinic Acetylcholine Receptor, β2-Subunits, Monoclonal Antibody
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文献和实验Nicotinic Receptor Systems and Neurobehavioral Function in Zebrafish
Nicotinic acetylcholine receptors appear to be quite ancient phylogenetically and are used in the nervous systems of a great number of species across broad parts of the animal kingdom. They play important roles in a variety of neurobehavioral
Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection
to the sequences of human and rat acetylcholine receptor subunits was immobilized on glass and silicon slides coated by a copolymer of N ,N -dimethylacrylamide, N -acryloyloxysuccinimide, and 3-(trimethoxysilyl) propyl methacrylate, copoly(DMA–NAS–MAPS
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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