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- R&D
- 美国/中国
- xy-37R
- 2025年07月16日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Neurofilament H Non-Phosphorylated (SMI 37) Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal Antibody against Non-Phosphorylated Neurofilament H Clone SMI-37 Form Ascites Fluid (contains 0.01M sodium azide) Host Mouse Species Reactivity Mammalian IsoType Neurofilament H Non-Phosphorylated (SMI 37) Monoclonal AntibodyIgM Specificity SMI 37 reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. Immunocytochemically, SMI 37 visualizes some neuronal cell bodies, dendrites and thick axons in the central and peripheral nervous systems. SMI 37 staining is more restricted than that seen with SMI 32 or SMI 38 and Neuronal cell bodies are intensely stained while neuronal processes staining is less prominent. This IgM antibody reacts with goat antimouse IgG and mouse ClonoPAP® via the light chains common to IgG and IgM. Uses This antibody is effective in immunoblotting, immunocytochemistry and ELISA. Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. - Western blot: 1:1,000
- Immunohistochemistry: 1:1,000
- ELISA: 1:1,000
Neurofilament H Non-Phosphorylated (SMI 37) Monoclonal AntibodyThe extent of permissible dilution of SMI 37 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
Tissue Preparation: SMI 37 reacts on Western blots, paraffin, vibratome and frozen tissue sections as well as cell cultures. Tissues and cultures can be fixed in a variety of paraformaldehyde or formaldehyde-containing fixatives such as Bouins. Reaction is poor in material fixed in glutaraldehyde/paraformaldehyde. In formalin-fixed, paraffin-embedded tissue, reaction with SMI 37 can be enhanced by autoclaving deparaffinized sections for 10 min in distilled water (Shin et al, Lab Invest, 64:693, 1991). Trypsin pretreatment abolishes reactivity with these antibodies. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of SMI 37 to neurons in frozen sections or thick sections of tissue perfused with 4% paraformaldehyde and in tissue cultures.Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Neurofilament H Non-Phosphorylated (SMI 37) Monoclonal Antibody
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pellet gently by tapping the tube. Volume should be approx. 0.8 ml. Set a timer. Add an equal volume of PEG solution, slowly, dropwise, with gentle tapping, over 1.5 min. at room temp. Then gently wiggle the tube for 1.5 min.at 37°C. Some cell clumping
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Neurofilament H Non-Phosphorylated (SMI 37) Monoclonal Antibody
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