- 询价
- R&D
- 美国/中国
- xy-39620
- 2025年07月15日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Prion (3F4) Monoclonal Antibody, Purified (SIGNET) 详细信息：
-
Description Exclusively Provided by Covance - SIGNET ANTIBODIES" height="1" src="https://store.crpinc.com/images/spacer.gif" style="list-style: none; padding: 0px; margin: 0px; border: 0px;" width="5"> Monoclonal Antibody against Prion Protein (3F4)
(Formerly Signet Catalog Nos: 9620-02, 9620-05, 9620-10)
Covance is the Exclusive Provider of this Antibody ProductIntended Use 
**Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone 3F4 Form Purified Antibody (in PBS) Host Prion (3F4) Monoclonal Antibody, Purified (SIGNET)Mouse Species Reactivity Human, Hamster, Feline IsoType IgG2a [Ab] Exclusively Provided by Covance - SIGNET ANTIBODIES" height="1" src="https://store.crpinc.com/images/spacer.gif" style="list-style: none; padding: 0px; margin: 0px; border: 0px;" width="5"> 2 mg/mL Specificity Exclusively Provided by Covance - SIGNET ANTIBODIES" height="1" src="https://store.crpinc.com/images/spacer.gif" style="list-style: none; padding: 0px; margin: 0px; border: 0px;" width="5"> 3F4 is reactive to amino acid residues 109-112 of prion protein (PrP) from humans, hamsters, and felines. It recognizes both protease sensitive and protease resistant forms of PrP (after denaturing). Uses Exclusively Provided by Covance - SIGNET ANTIBODIES" height="1" src="https://store.crpinc.com/images/spacer.gif" style="list-style: none; padding: 0px; margin: 0px; border: 0px;" width="5"> This antibody is effective in immunoblotting, immunohistochemistry (IHC) on unfixed tissues, immunoprecipitation (IP) and ELISA. Suggested Working Dilution Exclusively Provided by Covance - SIGNET ANTIBODIES" height="1" src="https://store.crpinc.com/images/spacer.gif" style="list-style: none; padding: 0px; margin: 0px; border: 0px;" width="5"> The optimal working dilution should be determined for each specific assay condition. - WB: 1:10,000 - 1:100,000 (Refs 2, 4)
- IHC: 1:100 - 1:1,000 (Refs 2, 4)
Pretreatment: Epitope must be re-exposed in fixed tissue by pretreatment of tissue using one of the following procedures:
1. 70% Formic acid for 10-30 min at RT (Ref 1)
2. Hydrolytic autoclaving (Ref 2)
- IP: 1:10-1:100 (Ref 3, 4)
- ELISA: 1:100,000- 1:1,000,000 (Ref 2, 4)
Storage Exclusively Provided by Covance - SIGNET ANTIBODIES" height="1" src="https://store.crpinc.com/images/spacer.gif" style="list-style: none; padding: 0px; margin: 0px; border: 0px;" width="5"> Prion (3F4) Monoclonal Antibody, Purified (SIGNET)Do not store antibody diluted below 50 ug/mL in the absence of protein (i.e.: add 2% bovine serum albumin). It is suggested that the total volume is aliquoted upon initial thaw. The antibody is best stored undiluted at -20°C to -70°C. References 1. Kondoh G, et al. Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization. Nat Med 11(2):160-66, 2005. (IHC)
2. Gilch S, et al. Recognition of lumenal prion proetin aggregates by post-ER quality control mechanisms is mediated by the preoctarepeat region of PrP. Traffic 5:300-313, 2004. (WB, IHC, ELISA)
3. Nishina K, et al. In vitro prion protein conversion in detergent solubilized membranes. Biochem 43:2613-2621, 2004. (IP)
4. Shiga Y, et al. Diffusion-weighted MRI abnormalities as an early marker for Creutzfeldt-Jakob disease. Neurol 63:443-449, 2004. (WB, IHC, IP, ELISA)
5. Zou W, et al. Antibody to DNA detects scrapie but not normal prion protein. PNAS 101(5):1380-1385, 2004.
6. Kitamoto T, et al. Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab Invest 57:230-236, 1987.
7. Kascsak, et al. Mouse polyclonal and monoclonal antibody to scrapie associated fibril proteins. J Virol 61:3688-3693, 1987.Warranty/Conditions Exclusively Provided by Covance - SIGNET ANTIBODIES" height="1" src="https://store.crpinc.com/images/spacer.gif" style="list-style: none; padding: 0px; margin: 0px; border: 0px;" width="5"> Covance products may not be resold or modified for resale without prior written approval. - Prion (3F4) Monoclonal Antibody, Purified (SIGNET)
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Prion (3F4) Monoclonal Antibody, Purified (SIGNET)
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