- 询价
- R&D
- 美国/中国
- xy-32380
- 2025年07月13日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Tris-Buffered Saline 20X (1.0 M) w/ Tween 20 (SIGNET)保存于运输说明：
- Store this product at room temperature
- 详细信息：
-
Description 
Tris-Buffered Saline 20X (1.0 M) w/ Tween 20
(Formerly Signet Catalog No.: 2380)Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Form Tris-Buffered Saline 20X (1.0 M) w/ Tween 20 (SIGNET)Buffer Specificity 500mL of TBS 20X w/Tween 20 concentrate is sufficient for preparing 10 liters of 50mM Tris-Buffered Saline, pH 7.6 Uses Signets TBS 20X (1.0M) concentrate is used to prepare buffered saline solutions that are utilized in many immunohistochemical procedures. Notes Prepare the TBS solution by combining components as described in the following table.
Buffer = 20X TBS + DI Water
1L = 50mL + 950mL
5L = 250mL + 4750mL
10L = 500mL + 9500mLStorage Tris-Buffered Saline 20X (1.0 M) w/ Tween 20 (SIGNET)Store this product at room temperature. Unopened 20X (1.0M) w/Tween 20 is stable 1 year at RT. TBS solutions that become turbid should be discarded. Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Tris-Buffered Saline 20X (1.0 M) w/ Tween 20 (SIGNET)
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文献和实验-Cl, pH8.0 1.2114 20% (v/v) Glycerol 20g 4% (w/v) SDS 4g 0.2% Bromophenol blue 0.2g 200mM DTT 20ul 5x Running buffer: 1 L 15.1 g Trizma base (= 125m M) 94g Glycine (= 1.25 M) 50ml 10%SDS (= 0.5%)--add last Do not adjust the pH!! 10x Blotting
Replication timing by density transfer
Calbiochem's Pronase (catalog #53702), which seems pretty clean--i.e., plates spread after Pronase treatment aren't contaminated--but I heard from someone who'd used Sigma's Pronase and had massive growth of Streptomyces. 20x SCP = 0.6 M Na2 HPO
Clonality - X Chromosome Inactivation Assay
can be utilized. 1. Materials DNA sample (see Processing of Microdissected Tissue - DNA-based Analysis) Proteinase K (Sigma) Proteinase K buffer (0.05 M Tris-HCL, 0.001 M EDTA, 1% Tween 20, 0.1 mg/ml proteinase K, pH 8.0) Phenol:chloroform:isoamyl alcohol
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