- 询价
- R&D
- 美国/中国
- xy-35R
- 2025年07月07日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
-20℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
- Neurofilaments, Hypophosphorylated (SMI 35) Monoclonal Antibody保存于运输说明：
- Store at -20°C
- 详细信息：
-
Description 
Monoclonal Antibody to Hypo-Phosphorylated Neurofilaments Intended Use **Research Use Only (RUO)**
This product is sold for laboratory research use only, not for human or in-vivo use.Clone SMI-35 Form Ascites Fluid Host Mouse Species Reactivity Mammalian IsoType Neurofilaments, Hypophosphorylated (SMI 35) Monoclonal Antibody IgG1 Specificity While other antiphosphoneurofilaments react with neurofilaments of high degrees of phosphorylation, SMI 35 reacts with neurofilaments of low degrees of phosphorylation as well. On two-dimensional immumoblots, it detects a band extending from the phosphorylated neurofilament position at 200kDa (pI 5.1) toward the non-phosphorylated position at 170kDa (pI 6.2). It reacts primarily with neurofilament H and, to a lesser extent, with neurofilament M in most mammalian species, as well as in chicken and frog (Xenopus). This antibody stains thick and thin axons and faintly stains cell bodies. It reveals an especially rich network of thin axons. Other cells and tissues are unreactive except for peripheral axons. In line with the low degree of neurofilament phosphorylation sufficient for reacion with SMI 35, more extensive phosphatase treatment of neurofilaments is necessary for abolition of reaction with this antibody than with any other antibody to phosphorylated neurofilaments. In pathological conditions, SMI 35 frequently detects early changes in neuronal cell body staining. A Golgi-like image of pathologic phosphorylation in neuronal perikarya and their dendritic arborizations has been obtained by ClonoPAP immunocytochemistry with SMI 35 providing a pre-chromatolytic, retrograde demonstration of neuronal connectivities. SMI 35 reacts with occasional intraneuronal tangles in Alzheimers disease. This reaction increases in scope and degree with mild dephosphorylation but is abolished by extensive dephosphorylation. Uses This antibody is effective in immunoblotting, immunocytochemistry and ELISA. This antibody can be used for neurofilament detection in trypsin-treated tissue. Suggested Working Dilution Neurofilaments, Hypophosphorylated (SMI 35) Monoclonal Antibody The optimal working dilution should be determined for each specific assay condition. - Western blot: 1:1,000*
- Immunohistochemistry: 1:1,000
- ELISA: 1:5,000-10,000
The extent of permissible dilution of SMI 35 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined.
Tissue Preparation: SMI 35 reacts on Western blots, paraffin, vibratome and frozen tissue sections as well as with neuronal cell cultures. Tissues and cultures can be fixed in a variety of paraformaldehyde or formaldehyde-containing fixatives including Bouins. "Recovery" of antigen in formalin-fixed tissue can be accomplished by autoclaving of deparaffinized sections in distilled water (Shin et al, Lab Invest, 64:693, 1991) or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min (Evers and Uylings, J Neurosci Methods 72:197, 1997). Post-fixation in cold methanol greatly facilitates access of SMI 35 in cryostat or thick tissue sections and in tissue cultures.Notes *On 2 dimensional gels, this antibody detects a band extending from the phosphorylated neurofilament position at 200 kDa (pI 5.1) toward the non-phosphorylated position at 170kDa (pI 6.2) Storage Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. References Petzold A, Keir G, Green AJ, Giovannoni G, Thompson EJ. A specific ELISA for measuring neurofilament heavy chain phosphoforms. J Immunol Methods 278(1-2):179-90, 2003. (ELISA) Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval. - Neurofilaments, Hypophosphorylated (SMI 35) Monoclonal Antibody
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xy-4532xyK Clenbuterol/KLH克仑特罗偶联血蓝蛋白
xy-1566xy CRIPTO畸胎瘤衍化生长因子抗原(表皮生长因子相关肽)N端
xy-1028xy Cytokeratin 19细胞角蛋白19多肽抗原
xy-0719xy CEA(Carcinoembryonic Antigen )peptide人癌胚抗原抗原
xy-0579xy CD105CD105/转化生长因子β受体抗原
xy-0084xy Caspase-6 (NT)半胱胺酸蛋白酶蛋白-6（抗原）
xy-1448xy CD339/Jagged1CD339/Jagged1抗原
xy-0469xy C-fos即刻早期基因(一种原癌基因)抗原
xy-0765xy CD3 epsilonCD3 epsilon(抗原)
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xy-0971xyA chloramphenicol/OVA氯霉素偶联鸡卵白蛋白蛋白
xy-2552xy CXCL11干扰素诱导T细胞趋化因子抗原
xy-1598xy Cathepsin G/CTSG组织蛋白酶G
xy-0649xy CD68CD68（多肽抗原）
xy-0042xy ChAT(Choline Acetyltransferase)胆碱乙酰转移酶（抗原）
xy-1186xy CD36L1/SRB1高密度脂蛋白受体/清道夫受体抗原
xy-10066xy Cox A16 polymerase3D柯萨奇病毒A16型聚蛋白3D
xy-2141xy CRIPTO畸胎瘤衍化生长因子抗原(表皮生长因子相关肽)C端
xy-2042xy CD33CD33抗原（人）
xy-2961xy Calsequestrin肌钙集蛋白/收钙素
xy-2190xy Cytokeratin 19细胞角蛋白19抗原
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xy-4535Kxy DHT/KLH protein双氢睾酮偶联血蓝蛋白
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xy-4535Bxy DHT/BSA双氢睾酮偶联牛血清白蛋白
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Neurofilaments, Hypophosphorylated (SMI 35) Monoclonal Antibody
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