- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA248Ge
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 General，通用
牛血清白蛋白(BSA)检测试剂盒
检测范围 2.47-200ug/mL 灵敏度 0.87ug/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
牛血清白蛋白(BSA)检测试剂盒
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ybC888Ra 大鼠嗜铬蛋白B(CHGB)ELISA试剂盒 ELISA Kit for Chromogranin B (CHGB)
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ybB834Ra 大鼠凝血因子Ⅺ(F11)ELISA试剂盒 ELISA Kit for Coagulation Factor XI (F11)
ybB538Ra 大鼠细胞胱硫醚γ裂解酶(CHZ)ELISA试剂盒 ELISA Kit for Cystathionine Gamma LyaHZ (CHZ)
ybB320Ra 大鼠胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒 ELISA Kit for Thymic Stromal Lymphopoietin (TSLP)
ybA741Ra 大鼠Dickkopf相关蛋白1(DKK1)ELISA试剂盒 ELISA Kit for Dickkopf Related Protein 1 (DKK1)
ybF880Ra 大鼠分泌型卷曲相关蛋白1(SFRP1)ELISA试剂盒 ELISA Kit for HZcreted Frizzled Related Protein 1 (SFRP1)
ybC442Ra 大鼠双肾上腺皮质激素(DCX)ELISA试剂盒 ELISA Kit for Doublecortin (DCX)
ybE118Ra 大鼠肝配蛋白B受体3(EPHB3)ELISA试剂盒 ELISA Kit for Ephrin Type B ReHZptor 3 (EPHB3)
ybF609Ra 大鼠肌微管素相关蛋白9(MTMR9)ELISA试剂盒 ELISA Kit for Myotubularin Related Protein 9 (MTMR9)
ybF834Ra 大鼠转化受体电位阳离子通道亚家族M成员4(TRPM4)ELISA试剂盒 ELISA Kit for Transient ReHZptor Potential Cation Channel Subfamily M, Member 4 (TRPM4)
牛血清白蛋白(BSA)检测试剂盒
检测范围 2.47-200ug/mL 灵敏度 0.87ug/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
牛血清白蛋白(BSA)检测试剂盒
ELISA Kit for Bovine Serum Albumin (BSA)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | General |
| Product No. | CEA248Ge |
| Sample type | Serum, plasma and other biological fluids. |
| Format | 96T |
| Assay length | 2.5 hours |
| Detection range | 2.47-200ug/mL The standard curve concentrations used for the ELISA’s were 200ug/mL, 66.67ug/mL, 22.22ug/mL, 7.41ug/mL, 2.47ug/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.87ug/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine Serum Albumin (BSA).
No significant cross-reactivity or interference between Bovine Serum Albumin (BSA) and analogues was observed.
No significant cross-reactivity or interference between Bovine Serum Albumin (BSA) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Bovine Serum Albumin (BSA) and the recovery rates were calculated by comparing the measured value to the expected amount of Bovine Serum Albumin (BSA) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 87-96 | 93 |
| heparin plasma(n=5) | 81-102 | 92 |
Precision
牛血清白蛋白(BSA)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bovine Serum Albumin (BSA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bovine Serum Albumin (BSA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bovine Serum Albumin (BSA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bovine Serum Albumin (BSA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-94% | 80-96% | 87-103% | 93-101% |
| EDTA plasma(n=5) | 95-102% | 97-105% | 87-94% | 95-102% |
| heparin plasma(n=5) | 97-104% | 95-105% | 86-98% | 91-98% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
牛血清白蛋白(BSA)检测试剂盒This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Bovine Serum Albumin (BSA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Bovine Serum Albumin (BSA) and unlabeled Bovine Serum Albumin (BSA) (Standards or samples) with the pre-coated antibody specific to Bovine Serum Albumin (BSA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Bovine Serum Albumin (BSA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Bovine Serum Albumin (BSA) in the sample.
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ybC888Ra 大鼠嗜铬蛋白B(CHGB)ELISA试剂盒 ELISA Kit for Chromogranin B (CHGB)
ybD950Ra 大鼠孕烷X受体(PXR)ELISA试剂盒 ELISA Kit for Pregnane X ReHZptor (PXR)
ybD405Ra 大鼠肌肉磷酸果糖激酶(PFKM)ELISA试剂盒 ELISA Kit for PhosphofructokinaHZ, Muscle (PFKM)
ybB307Ra 大鼠纤维蛋白肽B(FPB)ELISA试剂盒 ELISA Kit for Fibrinopeptide B (FPB)
ybC746Ra 大鼠外周鞘磷脂蛋白22(PMP22)ELISA试剂盒 ELISA Kit for Peripheral Myelin Protein 22 (PMP22)
ybL969Ra 大鼠醌NADH脱氢酶1(NQO1)ELISA试剂盒 ELISA Kit for NADH DehydrogenaHZ, Quinone 1 (NQO1)
ybB834Ra 大鼠凝血因子Ⅺ(F11)ELISA试剂盒 ELISA Kit for Coagulation Factor XI (F11)
ybB538Ra 大鼠细胞胱硫醚γ裂解酶(CHZ)ELISA试剂盒 ELISA Kit for Cystathionine Gamma LyaHZ (CHZ)
ybB320Ra 大鼠胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒 ELISA Kit for Thymic Stromal Lymphopoietin (TSLP)
ybA741Ra 大鼠Dickkopf相关蛋白1(DKK1)ELISA试剂盒 ELISA Kit for Dickkopf Related Protein 1 (DKK1)
ybF880Ra 大鼠分泌型卷曲相关蛋白1(SFRP1)ELISA试剂盒 ELISA Kit for HZcreted Frizzled Related Protein 1 (SFRP1)
ybC442Ra 大鼠双肾上腺皮质激素(DCX)ELISA试剂盒 ELISA Kit for Doublecortin (DCX)
ybE118Ra 大鼠肝配蛋白B受体3(EPHB3)ELISA试剂盒 ELISA Kit for Ephrin Type B ReHZptor 3 (EPHB3)
ybF609Ra 大鼠肌微管素相关蛋白9(MTMR9)ELISA试剂盒 ELISA Kit for Myotubularin Related Protein 9 (MTMR9)
ybF834Ra 大鼠转化受体电位阳离子通道亚家族M成员4(TRPM4)ELISA试剂盒 ELISA Kit for Transient ReHZptor Potential Cation Channel Subfamily M, Member 4 (TRPM4)
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文献和实验相关实验
相关专题 牛血清白蛋白(BSA)，又称第五组分，是牛血清中的一种球蛋白，包含583个氨基酸残基，分子量为66.430 kDa，等电点为4.7。牛血清白蛋白在生化实验中有广泛的应用，例如在western blot 中作为Blocking agent。 用途 1、用于生化研究、遗传工程和医药研究 2、用作医药保健食品、调味品 3、维持渗透压、pH缓冲、载体作用
相关专题 牛血清白蛋白(BSA)，又称第五组分，是牛血清中的一种球蛋白，包含583个氨基酸残基，分子量为66.430 kDa，等电点为4.7。牛血清白蛋白在生化实验中有广泛的应用，例如在western blot 中作为Blocking agent。 用途 1、用于生化研究、遗传工程和医药研究 2、用作医药保健食品、调味品 3、维持渗透压、pH缓冲、载体 作用 4、在PCR
如何选择合适的牛血清白蛋白(BSA)？ 由于牛血清白蛋白(BSA)具有多种产品样式，可满足不同种的应用。目前网上的信息大多模棱两可，有牛血清白蛋白，也有牛血清白蛋白v组分等，很多 供货商对其区别也是不清楚的；另外，市场上还提供无蛋白酶、无DNase及无RNase、无脂肪酸、微生物级、试剂级、诊断分析级等不同等BSA粉末，因 此如何选择合适的BSA产品是很重要的前提。一般来说，普通
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牛血清白蛋白(BSA)检测试剂盒
¥900 - 1890
