- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA544Ga
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Chicken (Gallus，鸡)
免疫球蛋白G(IgG)检测试剂盒
检测范围 1.23-100ug/mL 灵敏度 0.48ug/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
免疫球蛋白G(IgG)检测试剂盒
ybA442Ra 大鼠阻抑素(PHB)ELISA试剂盒 ELISA Kit for Prohibitin (PHB)
ybA642Ra 大鼠果糖二磷酸醛缩酶A(ALDOA)ELISA试剂盒 ELISA Kit for AldolaHZ A, FructoHZ Bisphosphate (ALDOA)
ybD319Ra 大鼠果糖二磷酸醛缩酶B(ALDOB)ELISA试剂盒 ELISA Kit for AldolaHZ B, FructoHZ Bisphosphate (ALDOB)
ybD320Ra 大鼠果糖二磷酸醛缩酶C(ALDOC)ELISA试剂盒 ELISA Kit for AldolaHZ C, FructoHZ Bisphosphate (ALDOC)
ybD761Ra 大鼠谷氨酰胺合成酶(GS)ELISA试剂盒 ELISA Kit for Glutamine synthetaHZ (GS)
ybB276Ra 大鼠激素敏感性脂肪酶(LIPE)ELISA试剂盒 ELISA Kit for LipaHZ, Hormone HZnsitive (LIPE)
ybB083Ra 大鼠线粒体超氧化物歧化酶(SOD2)ELISA试剂盒 ELISA Kit for Superoxide DismutaHZ 2, Mitochondrial (SOD2)
ybD058Ra 大鼠中性鞘磷脂酶(NSMAHZ)ELISA试剂盒 ELISA Kit for Neutral SphingomyelinaHZ (NSMAHZ)
ybE912Ra 大鼠沉默调节蛋白1(SIRT1)ELISA试剂盒 ELISA Kit for Sirtuin 1 (SIRT1)
ybE913Ra 大鼠沉默调节蛋白3(SIRT3)ELISA试剂盒 ELISA Kit for Sirtuin 3 (SIRT3)
ybH337Ra 大鼠过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)ELISA试剂盒 ELISA Kit for Peroxisome Proliferator Activated ReHZptor Gamma Coactivator 1 Alpha (PPARgC1a)
ybB085Ra 大鼠肠碱性磷酸酶(ALPI)ELISA试剂盒 ELISA Kit for Alkaline PhosphataHZ, Intestinal (ALPI)
ybC033Ra 大鼠热休克蛋白β8(HSPβ8)ELISA试剂盒 ELISA Kit for Heat Shock Protein Beta 8 (HSPb8)
ybK477Ra 大鼠跨膜蛋白27(TMEM27)ELISA试剂盒 ELISA Kit for Transmembrane Protein 27 (TMEM27)
ybA536Ra 大鼠亮氨酰氨基肽酶(LAP)ELISA试剂盒 ELISA Kit for Leucine AminopeptidaHZ (LAP)
ybB693Ra 大鼠脂肪细胞型脂肪酸结合蛋白(FABP4)ELISA试剂盒 ELISA Kit for Fatty Acid Binding Protein 4, Adipocyte (FABP4)
ybF550Ra 大鼠核纤层蛋白A/C(LMNA)ELISA试剂盒 ELISA Kit for Lamin A/C (LMNA)
ybF209Ra 大鼠氨乙酰丙酸δ合酶1(ALAS1)ELISA试剂盒 ELISA Kit for Aminolevulinate Delta SynthaHZ 1 (ALAS1)
ybF417Ra 大鼠脂肪酸去饱和酶2(FADS2)ELISA试剂盒 ELISA Kit for Fatty Acid DesaturaHZ 2 (FADS2)
ybC468Ra 大鼠脂肪酸去饱和酶1(FADS1)ELISA试剂盒 ELISA Kit for Fatty Acid DesaturaHZ 1 (FADS1)
ybC669Ra 大鼠核呼吸因子1(NRF1)ELISA试剂盒 ELISA Kit for Nuclear Respiratory Factor 1 (NRF1)
ybL534Ra 大鼠生长停滞DNA损伤可诱导蛋白α(GADD45α)ELISA试剂盒 ELISA Kit for Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)
ybC935Ra 大鼠促肾上腺皮质素释放激素受体1(CRHR1)ELISA试剂盒 ELISA Kit for Corticotropin Releasing Hormone ReHZptor 1 (CRHR1)
免疫球蛋白G(IgG)检测试剂盒
检测范围 1.23-100ug/mL 灵敏度 0.48ug/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 2.5h 实验方法 竞争抑制法
规格 96T
免疫球蛋白G(IgG)检测试剂盒
ELISA Kit for Immunoglobulin G (IgG)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Chicken (Gallus) |
| Product No. | CEA544Ga |
| Sample type | Serum, plasma and other biological fluids. |
| Format | 96T |
| Assay length | 2.5 hours |
| Detection range | 1.23-100ug/mL The standard curve concentrations used for the ELISA’s were 100ug/mL, 33.33ug/mL, 11.11ug/mL, 3.7ug/mL, 1.23ug/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.48ug/mL. |
Specificity
免疫球蛋白G(IgG)检测试剂盒This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG).
No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.
No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Immunoglobulin G (IgG) and the recovery rates were calculated by comparing the measured value to the expected amount of Immunoglobulin G (IgG) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-105 | 101 |
| EDTA plasma(n=5) | 89-96 | 93 |
| heparin plasma(n=5) | 98-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Immunoglobulin G (IgG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-94% | 93-102% | 88-104% | 86-103% |
| EDTA plasma(n=5) | 99-105% | 97-105% | 85-96% | 81-98% |
| heparin plasma(n=5) | 94-103% | 81-98% | 82-95% | 91-99% |
Stability
免疫球蛋白G(IgG)检测试剂盒The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin G (IgG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample.
ybA442Ra 大鼠阻抑素(PHB)ELISA试剂盒 ELISA Kit for Prohibitin (PHB)
ybA642Ra 大鼠果糖二磷酸醛缩酶A(ALDOA)ELISA试剂盒 ELISA Kit for AldolaHZ A, FructoHZ Bisphosphate (ALDOA)
ybD319Ra 大鼠果糖二磷酸醛缩酶B(ALDOB)ELISA试剂盒 ELISA Kit for AldolaHZ B, FructoHZ Bisphosphate (ALDOB)
ybD320Ra 大鼠果糖二磷酸醛缩酶C(ALDOC)ELISA试剂盒 ELISA Kit for AldolaHZ C, FructoHZ Bisphosphate (ALDOC)
ybD761Ra 大鼠谷氨酰胺合成酶(GS)ELISA试剂盒 ELISA Kit for Glutamine synthetaHZ (GS)
ybB276Ra 大鼠激素敏感性脂肪酶(LIPE)ELISA试剂盒 ELISA Kit for LipaHZ, Hormone HZnsitive (LIPE)
ybB083Ra 大鼠线粒体超氧化物歧化酶(SOD2)ELISA试剂盒 ELISA Kit for Superoxide DismutaHZ 2, Mitochondrial (SOD2)
ybD058Ra 大鼠中性鞘磷脂酶(NSMAHZ)ELISA试剂盒 ELISA Kit for Neutral SphingomyelinaHZ (NSMAHZ)
ybE912Ra 大鼠沉默调节蛋白1(SIRT1)ELISA试剂盒 ELISA Kit for Sirtuin 1 (SIRT1)
ybE913Ra 大鼠沉默调节蛋白3(SIRT3)ELISA试剂盒 ELISA Kit for Sirtuin 3 (SIRT3)
ybH337Ra 大鼠过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)ELISA试剂盒 ELISA Kit for Peroxisome Proliferator Activated ReHZptor Gamma Coactivator 1 Alpha (PPARgC1a)
ybB085Ra 大鼠肠碱性磷酸酶(ALPI)ELISA试剂盒 ELISA Kit for Alkaline PhosphataHZ, Intestinal (ALPI)
ybC033Ra 大鼠热休克蛋白β8(HSPβ8)ELISA试剂盒 ELISA Kit for Heat Shock Protein Beta 8 (HSPb8)
ybK477Ra 大鼠跨膜蛋白27(TMEM27)ELISA试剂盒 ELISA Kit for Transmembrane Protein 27 (TMEM27)
ybA536Ra 大鼠亮氨酰氨基肽酶(LAP)ELISA试剂盒 ELISA Kit for Leucine AminopeptidaHZ (LAP)
ybB693Ra 大鼠脂肪细胞型脂肪酸结合蛋白(FABP4)ELISA试剂盒 ELISA Kit for Fatty Acid Binding Protein 4, Adipocyte (FABP4)
ybF550Ra 大鼠核纤层蛋白A/C(LMNA)ELISA试剂盒 ELISA Kit for Lamin A/C (LMNA)
ybF209Ra 大鼠氨乙酰丙酸δ合酶1(ALAS1)ELISA试剂盒 ELISA Kit for Aminolevulinate Delta SynthaHZ 1 (ALAS1)
ybF417Ra 大鼠脂肪酸去饱和酶2(FADS2)ELISA试剂盒 ELISA Kit for Fatty Acid DesaturaHZ 2 (FADS2)
ybC468Ra 大鼠脂肪酸去饱和酶1(FADS1)ELISA试剂盒 ELISA Kit for Fatty Acid DesaturaHZ 1 (FADS1)
ybC669Ra 大鼠核呼吸因子1(NRF1)ELISA试剂盒 ELISA Kit for Nuclear Respiratory Factor 1 (NRF1)
ybL534Ra 大鼠生长停滞DNA损伤可诱导蛋白α(GADD45α)ELISA试剂盒 ELISA Kit for Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a)
ybC935Ra 大鼠促肾上腺皮质素释放激素受体1(CRHR1)ELISA试剂盒 ELISA Kit for Corticotropin Releasing Hormone ReHZptor 1 (CRHR1)
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文献和实验相关实验
是血清中免疫球蛋白主成分，约占血清中免疫球蛋白总含量的75％。其中40～50％分布于血清中，其余分布在组织中。分子量约为150000道尔顿。人类血清中的IgG主要为单体，正常人的IgG包括四个亚型，其IgG1 占60～70％，IgG2 占15～20％，IgG3 占5～10％，IgG4 占1～7％。这些亚型在补体激活的经典途中结合能力各不相同。IgG主要由脾脏和淋巴结中的浆细胞合成，是唯一能通过胎盘的抗体，对防止新生儿出生数周内的感染起很大作用。婴儿初生后第3个月已能合成
Purification of Immunoglobulin G (IgG)
may consist of purification of total IgG or subpopulations (e.g., subclasses) of IgG from antisera/ascitic fluid/culture supernatant, or the isolation of a particular antigen binding fraction from such fluids. The former can be achieved by biochemical
正常参考值： IgG 7.0～16.6克/升（7.0～16.6g/L） 临床意义：（1） IgG增高： ① IgG、IgA、IgM在机体的防御中发挥重要作用。若IgG、IgA、IgM几种不同的免疫球蛋白均增高称之为多克隆性增高，常见于各种感染，特别是慢性感染、自身免疫性疾病如系统性红斑狼疮（SLE）、淋巴瘤、肺结核、肝脏疾病和寄生虫病等；医学教育|网搜集整理② 单一的免疫球蛋白增高，又称单克隆性增高，主要见于免疫增殖性疾病，如多发性骨髓瘤等。 （2） IgG降低： 可见于
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免疫球蛋白G(IgG)检测试剂盒
¥900 - 1890
