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- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
-20℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
- Rat PAP保存于运输说明:
- Store at -20°C
- 详细信息:
-
Description 
Peroxidase-antiperoxidase complex consisting of horseradish peroxidase and immunospecifically pure rat antiperoxidase. Form 
Purified Antibody (in PBS, 0.01M sodium azide) Host 
Rat [Ab] 
Rat PAP 4.86 mg/mL consisting of peroxidase and antiperoxidase Specificity 
Rat PAP is intended for the detection of monoclonal or serum-derived rat antibodies via an excess of an antirat IgG (such as goat antirat IgG Cat # SMI 5040). Rat antibodies of any isotype are detectable via reaction with shared light chains.
Composition: Immune complex of horseradish peroxidase and polyclonal rat antiperoxidase providing maximal immunocytochemical staining intensity at a dilution range of 1:100 to 1:1600. Staining intensity may decrease at dilutions of 1:3,200 and beyond.Uses 
This antibody is primarily used for immunohistochemistry applications, but it is also effective in blotting and ELISA assays. It can be used with diaminobenzidine tetrahydrochloride (DAB) substrates for detection with peroxidase. Suggested Working Dilution 
Rat PAP The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval. Because of the higher activity of our newly developed PAP, staining intensity is increased and more extensive dilutions are required with consequently greater economy. Dilution for use in immunocytochemistry depends on tissue preparation, abundance of antigen and quality of the first antibody. Therefore, we recommend that initially PAP be applied in a series of dilutions ranging from 1:100 to 1:1,600. Similar dilutions are recommended for Western blots and ELISA. Concentrations higher than those recommended for PAP or other antibody-based reagents may produce a brown background and decrease specific staining intensity. Storage 
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Warranty/Conditions 
Covance products may not be resold or modified for resale without prior written approval. - Rat PAP
- xy-1743xy DRD3 receptor(dopamine D3 receptor)多巴胺受体-D3抗原
xy-1746xy DRD4 receptor peptide多巴胺受体-D4抗原
xy-1747xy DRD5 receptor(dopamine D5 receptor)多巴胺受体-D5抗原
xy-0596xy DBH (Dopamine- Beta-Hydroxylase)多巴胺β羟化酶(抗原)
xy-0592xy DCC (Deleted in colorectal cancer gene)结直肠癌缺失基因抗原
xy-1269xy DCX(Doublecortin)双皮质素抗原
xy-2599xy DDB2(DNA damage-binding protein 2)损伤DNA结合蛋白2抗原
xy-1163xy Defensin Beta1防御素β1抗原
xy-2165xy Defensin Beta1(human)防御素β1抗原(人)
xy-1295xy Defensin Beta2/beta-defensin 2/BD-2(mouse)防御素β2抗原(小鼠)
xy-1296xy Defensin Beta2/beta-defensin 2/BD-2(rat)防御素β2抗原(大鼠)
xy-1687xy DAPK1(Death associated protein kinase 1)凋亡相关蛋白激酶1抗原
xy-2197xy DHAV(duck hepatitis A virus)鸭甲型肝炎A病毒抗原
xy-1026xy Des(Desmin)结蛋白抗原
xy-2162xy DKK1(Dickkopf 1)抑癌蛋白DKK1抗原
xy-0595xy DMBT1 (Deleted in malignant brain tumor 1)DMBT1抑癌基因抗原
xy-1359xy DNA-PKcs peptideDNA依赖蛋白激酶催化亚基多肽抗原
xy-0597xy DPPA2 (developmental pluripotency associated gene2)多能发育相关基因2抗原
xy-0591xy DR4/APO2/TRAILR1 (Death receptor 4)死亡受体4抗原
xy-1696xy DR5/TNFRSF10B/CD262 (Death receptor 5)肿瘤细胞调亡素/死亡受体5抗原
xy-1748xy DP-I/DSP(desmoplakin I )桥粒斑蛋白1抗原
xy-0598xy DVL1 (dishevelled 1)蓬乱蛋白1
xy-0574xy Dynamin 2(Anti-Human Dynamin-2 )鸟苷三磷酸酶-发动蛋白2抗体
xy-0831xy Nadrin peptide发育调节蛋白多肽抗原
xy-0599xy E2F1转录因子E2F-1
xy-1001xy E2F2转录因子E2F-2抗原
xy-1399xy E2F4转录因子E2F-4抗原
xy-1750xy E2F6转录因子E2F-6抗原
xy-2071xy E2 tagE2 tag多肽抗原
xy-1752xy EAAT3(Excitatory amino acid transporters 3)胶质细胞谷氨酸运载蛋白3抗原
xy-0820xy EBNA-3 (Epstein barr nuclear antigens 3)EB病毒编码核蛋白-3
xy-1190xy ECE1(Endothelin Converting Enzyme 1)内皮素转化酶1抗原
xy-1753xy ECE2(Endothelin Converting Enzyme 2)内皮素转化酶2抗原
xy-1754xy ECP(eosinophil cationic protein)mouse rat嗜酸性粒细胞阳离子蛋白抗原
xy-1149xy ED-1 (Ectodermal Dysplasia 1)外胚层发育不良抗原
xy-1755xy EGFL7(EGF-like domain containing protein 7)类表皮生长因子域7抗原
xy-1076xy Egr-1(Early growth response 1)早期反应基因-1/应急反应生长基因1抗原
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文献和实验The Peroxidase-Antiperoxidase (PAP) Method
The peroxidase-antiperoxidase (PAP) method was pioneered by Sternberger in 1979 (1 ). The method uses an immunological sandwich amplification and the enzyme peroxidase to effect a signal. The unique feature of this procedure is the enzyme
The PeroxidaseAntiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
Immunoenzyme procedures take on many forms, including, simply, antibody coupled to enzyme. These direct techniques require the labeling of all the primary antibodies and can produce more background. A more economical method uses a secondary
The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods
and indirect techniques, while still used today, were found to be less sensitive than the later-used peroxidase-antiperoxidase (PAP) techniques. The newer immune complex polymer type of detection system (Envision™ Dako Corporation, Santa Barbara, CA) increases
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