- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YBEA073Mu
- 2025年07月14日
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- 详细信息
- 询价记录
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Mus musculus (Mouse，小鼠)
白介素2(IL2)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 2.5pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
白介素2(IL2)检测试剂盒
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yb-E12830 大鼠肝细胞生长因子(HGF)ELISA试剂盒 Rat hepatocyte growth factor (HGF) ELISA kit
yb-E12831 大鼠糖蛋白130(gp130)ELISA试剂盒 Rats glycoprotein 130 (gp130) ELISA Kit
yb-E12832 大鼠生长激素(GH)ELISA试剂盒 Rat growth hormone (GH) ELISA kit
yb-E12833 大鼠粒细胞集落刺激因子(G-CSF)ELISA试剂盒 Cells Granulocyte colony-stimulating factor in rats (G-CSF) ELISA kit
yb-E12834 大鼠中性粒细胞趋化蛋白2(NAP-2/CXCL7)ELISA试剂盒 Rat neutrophil chemotactic protein 2 (NAP-2/CXCL7) ELISA kit
yb-E12835 大鼠FMS样酪氨酸激酶3(Flt3)ELISA试剂盒 Rat FMS -like tyrosine kinase 3 (Flt3) ELISA kit
yb-E12836 大鼠FMS样酪氨酸激酶3配体(Flt3L)ELISA试剂盒 Rat FMS -like tyrosine kinase 3 ligand (Flt3L) ELISA kit
yb-E12837 大鼠碱性成纤维细胞生长因子9(bFGF-9)ELISA试剂盒 Rat basic fibroblast growth factor 9 (bFGF-9) ELISA kit
yb-E12838 大鼠碱性成纤维细胞生长因子4(bFGF-4)ELISA试剂盒 Rat basic fibroblast growth factor 4 (bFGF-4) ELISA kit
yb-E12839 大鼠碱性成纤维细胞生长因子6(bFGF-6)ELISA试剂盒 Rat basic fibroblast growth factor 6 (bFGF-6) ELISA Kit
yb-E12840 大鼠酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒 Rat fibroblast growth factor 1 (aFGF-1) ELISA Kit
yb-E12841 大鼠凋亡相关因子配体(FASL) ELISA试剂盒 Rat apoptosis-related factor ligand (FASL) ELISA kit
yb-E12842 大鼠凋亡相关因子(FAS/CD95)ELISA试剂盒 Rat apoptosis-related factors (FAS/CD95) ELISA kit
yb-E12843 大鼠红细胞生成素(EPO)ELISA试剂盒 Rat Erythropoietin (EPO) ELISA kit
yb-E12844 大鼠红细胞生成素受体(EPOR)ELISA试剂盒 Rat erythropoietin receptor (EPOR) ELISA kit
yb-E12845 大鼠嗜酸粒细胞趋化蛋白Eotaxin 1(Eotaxin 1/CCL11)ELISA试剂盒 Rat eosinophil chemoattractant protein Eotaxin 1 (Eotaxin 1/CCL11) ELISA kit
yb-E12846 大鼠嗜酸粒细胞趋化因子(ECF)ELISA试剂盒 Rat eosinophil chemotactic factor (ECF) ELISA kit
yb-E12847 大鼠内分泌腺来源的血管内皮生长因子(EG-VEGF)ELISA试剂盒 Endocrine origin rat vascular endothelial growth factor (EG-VEGF) ELISA Kit
白介素2(IL2)检测试剂盒
检测范围 7.81-500pg/mL 灵敏度 2.5pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
白介素2(IL2)检测试剂盒
ELISA Kit for Interleukin 2 (IL2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | SEA073Mu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 7.81-500pg/mL The standard curve concentrations used for the ELISA’s were 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL, 7.81pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 2.5pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 2 (IL2).
No significant cross-reactivity or interference between Interleukin 2 (IL2) and analogues was observed.
No significant cross-reactivity or interference between Interleukin 2 (IL2) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 2 (IL2) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 2 (IL2) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-104 | 97 |
| EDTA plasma(n=5) | 92-101 | 97 |
| heparin plasma(n=5) | 78-102 | 92 |
Precision
白介素2(IL2)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 2 (IL2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 2 (IL2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-104% | 85-104% | 93-101% | 79-89% |
| EDTA plasma(n=5) | 78-101% | 95-103% | 80-97% | 80-89% |
| heparin plasma(n=5) | 83-101% | 91-105% | 78-89% | 84-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
白介素2(IL2)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 (IL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 (IL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 (IL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 2 (IL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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yb-E12828 大鼠胰岛素样生长因子结合蛋白1(IGFBP-1)ELISA试剂盒 Insulin -like growth factor binding protein- 1 (IGFBP-1) ELISA Kit
yb-E12829 大鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)ELISA试剂盒 Vascular endothelial growth factor receptor in rat 2 (VEGFR-2/Flk-1) ELISA kit
yb-E12830 大鼠肝细胞生长因子(HGF)ELISA试剂盒 Rat hepatocyte growth factor (HGF) ELISA kit
yb-E12831 大鼠糖蛋白130(gp130)ELISA试剂盒 Rats glycoprotein 130 (gp130) ELISA Kit
yb-E12832 大鼠生长激素(GH)ELISA试剂盒 Rat growth hormone (GH) ELISA kit
yb-E12833 大鼠粒细胞集落刺激因子(G-CSF)ELISA试剂盒 Cells Granulocyte colony-stimulating factor in rats (G-CSF) ELISA kit
yb-E12834 大鼠中性粒细胞趋化蛋白2(NAP-2/CXCL7)ELISA试剂盒 Rat neutrophil chemotactic protein 2 (NAP-2/CXCL7) ELISA kit
yb-E12835 大鼠FMS样酪氨酸激酶3(Flt3)ELISA试剂盒 Rat FMS -like tyrosine kinase 3 (Flt3) ELISA kit
yb-E12836 大鼠FMS样酪氨酸激酶3配体(Flt3L)ELISA试剂盒 Rat FMS -like tyrosine kinase 3 ligand (Flt3L) ELISA kit
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yb-E12839 大鼠碱性成纤维细胞生长因子6(bFGF-6)ELISA试剂盒 Rat basic fibroblast growth factor 6 (bFGF-6) ELISA Kit
yb-E12840 大鼠酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒 Rat fibroblast growth factor 1 (aFGF-1) ELISA Kit
yb-E12841 大鼠凋亡相关因子配体(FASL) ELISA试剂盒 Rat apoptosis-related factor ligand (FASL) ELISA kit
yb-E12842 大鼠凋亡相关因子(FAS/CD95)ELISA试剂盒 Rat apoptosis-related factors (FAS/CD95) ELISA kit
yb-E12843 大鼠红细胞生成素(EPO)ELISA试剂盒 Rat Erythropoietin (EPO) ELISA kit
yb-E12844 大鼠红细胞生成素受体(EPOR)ELISA试剂盒 Rat erythropoietin receptor (EPOR) ELISA kit
yb-E12845 大鼠嗜酸粒细胞趋化蛋白Eotaxin 1(Eotaxin 1/CCL11)ELISA试剂盒 Rat eosinophil chemoattractant protein Eotaxin 1 (Eotaxin 1/CCL11) ELISA kit
yb-E12846 大鼠嗜酸粒细胞趋化因子(ECF)ELISA试剂盒 Rat eosinophil chemotactic factor (ECF) ELISA kit
yb-E12847 大鼠内分泌腺来源的血管内皮生长因子(EG-VEGF)ELISA试剂盒 Endocrine origin rat vascular endothelial growth factor (EG-VEGF) ELISA Kit
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白介素2(IL2)检测试剂盒
¥900 - 1890
