- ¥900 - 1890
- 钰博生物
- 见说明书
- 德国/美国/中国
- YB437Hu01
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
转化生长因子β1(TGFβ1)检测试剂盒
检测范围 31.25-2000pg/mL 灵敏度 11.5pg/mL
样本类型 Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
转化生长因子β1(TGFβ1)检测试剂盒
yb-E11062 人可溶性磷脂酶A2(sPL-A2)ELISA试剂盒 Human soluble phospholipase A2 (sPL-A2) ELISA kit
yb-E11063 人脱氧核糖核酸酶Ⅰ(DNase-Ⅰ)ELISA试剂盒 Human DNase Ⅰ (DNase-Ⅰ) ELISA Kit
yb-E11064 人精氨酸酶(Arg)ELISA试剂盒 Human arginase (Arg) ELISA kit
yb-E11065 人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒 Human neutrophil alkaline phosphatase (NAP) ELISA kit
yb-E11066 人间变性淋巴瘤激酶(ALK)ELISA试剂盒 Human anaplastic lymphoma kinase (ALK) ELISA Kit
yb-E11067 人激动异构酶/细胞分裂素MB(CKMB)ELISA试剂盒 People excited isomerase / cytokinin MB (CKMB) ELISA kit
yb-E11068 人肌球蛋白轻链激酶(MLCK)ELISA试剂盒 Human myosin light chain kinase (MLCK) ELISA kit
yb-E11069 人谷胱甘肽硫转移酶pi基因(GSTpi)ELISA试剂盒 Human glutathione S-transferase pi gene (GSTpi) ELISA kit
yb-E11070 人谷胱甘肽S转移酶(GSTs)ELISA试剂盒 Human glutathione S-transferase (GSTs) ELISA kit
yb-E11071 人泛素分解酶(DUB)ELISA试剂盒 Human ubiquitin enzyme (DUB) ELISA kit
yb-E11072 人分泌型磷脂酶A2(sPLA2)ELISA试剂盒 Human secretory phospholipase A2 (sPLA2) ELISA kit
yb-E11073 人泛素连接酶(E3/UBPL)ELISA试剂盒 Human ubiquitin ligase (E3/UBPL) ELISA kit
yb-E11074 人肥大细胞类胰蛋白酶(MCT)ELISA试剂盒 Human mast cell tryptase (MCT) ELISA kit
yb-E11075 人多巴色素异构酶(DT)ELISA试剂盒 Human dopachrome isomerase (DT) ELISA Kit
yb-E11076 人泛素结合酶(E2/UBCE)ELISA试剂盒 Human ubiquitin conjugating enzyme (E2/UBCE) ELISA kit
yb-E11077 人蛋白质二硫键异构酶(PDI)ELISA试剂盒 Human protein disulfide isomerase (PDI) ELISA Kit
yb-E11078 人胱天蛋白酶激活的脱氧核糖核酸酶(CAD)ELISA试剂盒 Human caspase -activated DNase (CAD) ELISA kit
yb-E11079 人γ谷氨酰半胱氨酸合成酶(γ-ECS)ELISA试剂盒 Human γ -glutamylcysteine synthetase (γ-ECS) ELISA Kit
yb-E11080 人泛素激活酶(E1/UBAE)ELISA试剂盒 Human ubiquitin activating enzyme (E1/UBAE) ELISA kit
yb-E11081 人胃蛋白酶(PP)ELISA试剂盒 Human pepsin (PP) ELISA kit
yb-E11082 人己糖激酶(HK)ELISA试剂盒 People hexokinase (HK) ELISA kit
yb-E11083 人丙酮酸脱氢酶E1(PDH E1)ELISA试剂盒 Human pyruvate dehydrogenase E1 (PDH E1) ELISA Kit
yb-E11084 人糖原合成酶激酶(GSK)ELISA试剂盒 People glycogen synthase kinase (GSK) ELISA kit
yb-E11085 人胱天蛋白酶9(Casp-9)ELISA试剂盒 Human caspase 9 (Casp-9) ELISA Kit
yb-E11086 人胱天蛋白酶3(Casp-3)ELISA试剂盒 Human caspase 3 (Casp-3) ELISA Kit
yb-E11087 人中性粒细胞弹性蛋白酶(NE)ELISA试剂盒 Human neutrophil elastase (NE) ELISA Kit
yb-E11088 人碳酸酐酶2(CA-2)ELISA试剂盒 Human carbonic anhydrase 2 (CA-2) ELISA Kit
转化生长因子β1(TGFβ1)检测试剂盒
检测范围 31.25-2000pg/mL 灵敏度 11.5pg/mL
样本类型 Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
转化生长因子β1(TGFβ1)检测试剂盒
ELISA Kit for Transforming Growth Factor Beta 1 (TGFb1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA124Hu |
| Sample type | Serum, platelet-poor plasma, tissue homogenates, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 31.25-2000pg/mL The standard curve concentrations used for the ELISA’s were 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 11.5pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.
No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Transforming Growth Factor Beta 1 (TGFb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Transforming Growth Factor Beta 1 (TGFb1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-101 | 90 |
| EDTA plasma(n=5) | 92-99 | 96 |
| heparin plasma(n=5) | 81-103 | 101 |
Precision
转化生长因子β1(TGFβ1)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Transforming Growth Factor Beta 1 (TGFb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 98-105% | 97-105% | 92-101% |
| EDTA plasma(n=5) | 99-105% | 96-104% | 85-105% | 98-105% |
| heparin plasma(n=5) | 87-96% | 83-96% | 86-101% | 83-95% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
转化生长因子β1(TGFβ1)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 1 (TGFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1 (TGFb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E11061 人黏着斑激酶(FAK)ELISA试剂盒 Human focal adhesion kinase (FAK) ELISA kit
yb-E11062 人可溶性磷脂酶A2(sPL-A2)ELISA试剂盒 Human soluble phospholipase A2 (sPL-A2) ELISA kit
yb-E11063 人脱氧核糖核酸酶Ⅰ(DNase-Ⅰ)ELISA试剂盒 Human DNase Ⅰ (DNase-Ⅰ) ELISA Kit
yb-E11064 人精氨酸酶(Arg)ELISA试剂盒 Human arginase (Arg) ELISA kit
yb-E11065 人中性粒细胞碱性磷酸酶(NAP)ELISA试剂盒 Human neutrophil alkaline phosphatase (NAP) ELISA kit
yb-E11066 人间变性淋巴瘤激酶(ALK)ELISA试剂盒 Human anaplastic lymphoma kinase (ALK) ELISA Kit
yb-E11067 人激动异构酶/细胞分裂素MB(CKMB)ELISA试剂盒 People excited isomerase / cytokinin MB (CKMB) ELISA kit
yb-E11068 人肌球蛋白轻链激酶(MLCK)ELISA试剂盒 Human myosin light chain kinase (MLCK) ELISA kit
yb-E11069 人谷胱甘肽硫转移酶pi基因(GSTpi)ELISA试剂盒 Human glutathione S-transferase pi gene (GSTpi) ELISA kit
yb-E11070 人谷胱甘肽S转移酶(GSTs)ELISA试剂盒 Human glutathione S-transferase (GSTs) ELISA kit
yb-E11071 人泛素分解酶(DUB)ELISA试剂盒 Human ubiquitin enzyme (DUB) ELISA kit
yb-E11072 人分泌型磷脂酶A2(sPLA2)ELISA试剂盒 Human secretory phospholipase A2 (sPLA2) ELISA kit
yb-E11073 人泛素连接酶(E3/UBPL)ELISA试剂盒 Human ubiquitin ligase (E3/UBPL) ELISA kit
yb-E11074 人肥大细胞类胰蛋白酶(MCT)ELISA试剂盒 Human mast cell tryptase (MCT) ELISA kit
yb-E11075 人多巴色素异构酶(DT)ELISA试剂盒 Human dopachrome isomerase (DT) ELISA Kit
yb-E11076 人泛素结合酶(E2/UBCE)ELISA试剂盒 Human ubiquitin conjugating enzyme (E2/UBCE) ELISA kit
yb-E11077 人蛋白质二硫键异构酶(PDI)ELISA试剂盒 Human protein disulfide isomerase (PDI) ELISA Kit
yb-E11078 人胱天蛋白酶激活的脱氧核糖核酸酶(CAD)ELISA试剂盒 Human caspase -activated DNase (CAD) ELISA kit
yb-E11079 人γ谷氨酰半胱氨酸合成酶(γ-ECS)ELISA试剂盒 Human γ -glutamylcysteine synthetase (γ-ECS) ELISA Kit
yb-E11080 人泛素激活酶(E1/UBAE)ELISA试剂盒 Human ubiquitin activating enzyme (E1/UBAE) ELISA kit
yb-E11081 人胃蛋白酶(PP)ELISA试剂盒 Human pepsin (PP) ELISA kit
yb-E11082 人己糖激酶(HK)ELISA试剂盒 People hexokinase (HK) ELISA kit
yb-E11083 人丙酮酸脱氢酶E1(PDH E1)ELISA试剂盒 Human pyruvate dehydrogenase E1 (PDH E1) ELISA Kit
yb-E11084 人糖原合成酶激酶(GSK)ELISA试剂盒 People glycogen synthase kinase (GSK) ELISA kit
yb-E11085 人胱天蛋白酶9(Casp-9)ELISA试剂盒 Human caspase 9 (Casp-9) ELISA Kit
yb-E11086 人胱天蛋白酶3(Casp-3)ELISA试剂盒 Human caspase 3 (Casp-3) ELISA Kit
yb-E11087 人中性粒细胞弹性蛋白酶(NE)ELISA试剂盒 Human neutrophil elastase (NE) ELISA Kit
yb-E11088 人碳酸酐酶2(CA-2)ELISA试剂盒 Human carbonic anhydrase 2 (CA-2) ELISA Kit
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① 上交复旦Science 子刊揭示靶向 YY1 有望治疗糖尿病肾病转化生长因子β1（TGFβ1，由基因 TGFB1 编码）已被确定为糖尿病肾病产生的主要致病因子。然而，当前的拮抗 TGFβ1 的策略未能在临床试验中表现出有益的治疗结果。在一项新的研究中，来自中国上海交通大学和复旦大学等研究机构的研究人员为了鉴定出一种不同的治疗方法，设计出一种基于质谱的 DNA-蛋白相互作用筛选方法，以便发现与 TGFβ1 启动子结合的转录阻遏物。他们鉴定出一种称为阴阳 1（Yin Yang 1, YY
（Tumornecrosisfactor,TNF)根据其产生来源和结构不同，可分为TNF-α和TNF-β两类，前者由单核-巨噬细胞产生，后者由活化T细胞产生，又名淋巴毒素。TNF除具有杀伤肿瘤细胞外，还有免疫调节、参与发热和炎症的发生。 5，转化生长因子-β家族（Transforminggrowthfactor-βfamily,TGF-βfamily)主要包括TGF-β1、TGF-β2、TGF-β3、TGFβ1β2以及骨形成蛋白（BMP）等。 6.趋化因子家族（Chemokinefamily)包括两个亚族：（1）C-X-C
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转化生长因子β1(TGFβ1)检测试剂盒
¥900 - 1890
