- ¥900 - 1890
- 钰博生物
- 见说明书
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- YBEA092Hu
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1000
- 供应商：
上海钰博
- 检测范围：
见说明书
- 检测方法：
酶联免疫
- 应用：
科研
- 标记物：
见说明书
- 样本：
血清/组织/尿液
适用生物 Homo sapiens (Human，人)
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 5.7pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒
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巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒
检测范围 15.63-1000pg/mL 灵敏度 5.7pg/mL
样本类型 Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒
ELISA Kit for Macrophage Inflammatory Protein 1 Alpha (MIP1a)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEA092Hu |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5.7pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Alpha (MIP1a).
No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.
No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.
Recovery
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒Matrices listed below were spiked with certain level of recombinant Macrophage Inflammatory Protein 1 Alpha (MIP1a) and the recovery rates were calculated by comparing the measured value to the expected amount of Macrophage Inflammatory Protein 1 Alpha (MIP1a) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-93 | 90 |
| EDTA plasma(n=5) | 89-103 | 92 |
| heparin plasma(n=5) | 89-96 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 1 Alpha (MIP1a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Macrophage Inflammatory Protein 1 Alpha (MIP1a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-98% | 88-96% | 80-101% | 92-99% |
| EDTA plasma(n=5) | 94-105% | 91-104% | 85-92% | 80-95% |
| heparin plasma(n=5) | 88-96% | 80-101% | 98-105% | 93-104% |
Stability
巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Alpha (MIP1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Alpha (MIP1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Alpha (MIP1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验相关实验
小鼠 巨噬细胞炎性蛋白1α(MIP-1α) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 巨噬细胞炎性蛋白1α(MIP-1α) 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 小鼠 巨噬细胞炎性蛋白1 α (MIP-1 α ) 水平。用纯化的 小鼠 巨噬细胞炎性蛋白1 α (MIP-1 α ) 抗体包被微孔板，制成固相
免疫球蛋白 Fc 段受体 Fc α R FcαR 即CD89分子，包括a1、a2和a3等不同的异型，其中a1是跨膜形式，胞膜外区有2个IgSFC2样结构域。CD89分布于外周血和黏膜组织中绝大部分吞噬细胞，某些T细胞和B细胞亚群也表达CD89。a1异型表达于单核细胞和中性粒细胞，a2异型表达于肺泡巨噬细胞。CD89可与IgAl和IgA2 h段结合，CD89为IgA中亲和力受体，能结合血清型或分泌型IgAl和IgA2，介导吞噬细胞的吞噬作用、超氧产生、释放
性，图中提供的是原始数据而非对数值。推荐使用对数 值建立标准曲线图。由于实验操作条件的不同（如操作者、移液技术、洗板技术和温度条件等），标准曲线的 O.D. 值会有所差异。所提供的标准曲线仅供参考，实验者需要根据自已的实验建立标准曲线。人白介素 8 受体α（IL8Ra） 检测试剂盒标准曲线检测范围0.156-10ng/mL最低检测限0.072ng/mL 此值为 20 个空白样品 （即标准品稀释液） 测定的平均值加二倍标准差所对应的浓度。精密度精密度用样品测定值的变异系数 CV
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巨噬细胞炎性蛋白1α(MIP1α)检测试剂盒
¥900 - 1890
