- 询价
- R&D
- 美国/中国
- xy-0012
- 2025年07月08日
- 科研使用
- Goat，Rabbit，Mouse
- 人，大鼠，小鼠，兔
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液态/粉状
- 保存条件：
4℃保存
- 克隆性：
多克隆
- 标记物：
见说明书
- 适应物种：
人，大鼠，小鼠，兔
- 宿主：
Goat，Rabbit，Mouse
- 应用范围：
科研使用
- 浓度：
1mg/1ml
- 靶点：
来电咨询
Product Overview
The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cellspecific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. As human EC and ES cells undergo differentiation, expression of TRA-1- 81 antigen is lost. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Stemgent® Phycoerythrin (PE) anti-Human TRA-1-81 AntibodyProduct Specifications
Imunogen Human embryonal carcinoma cell line 2102Ep
Reactivity Human
Clone TRA-1-81
Isotype Mouse IgM, κ
Number of Tests 50
Formulation Phosphate-buffered solution, pH 7.2, containing ≤0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography.
Storage Store at 4°C. Do not freeze. Light sensitive.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by flow cytometry to ensure product quality.
Recommended Usage 20 μl (0.25 μg) per 1x106 viable cells
Stemgent® Phycoerythrin (PE) anti-Human TRA-1-81 AntibodyTechnical Documents
Specifications
09-0012 Specifications Sheet
MSDS
Material Safety Data Sheet
Related Products
No Related Products
Stemgent® Phycoerythrin (PE) anti-Human TRA-1-81 Antibody
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文献和实验Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
on the beads was detected using biotinylated antigen/streptavidin-phycoerythrin. The method was demonstrated by isotyping antibodies directed against an anti-CD11a antibody therapeutic (RAPTIVA� /efalizumab) from the serum of a cynomolgus monkey hyper-immunized
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