Stemgent® Phycoerythrin (PE) R

Stemgent® Phycoerythrin (PE) Rat anti-Human SSEA-3 Antibody详细信息：
Product Overview

The Stemgent® Phycoerythrin (PE) Rat anti-Human SSEA-3 Antibody was screened on human embryonic stem (ES) cells using flow cytometry (FC) and selected as the best stem cell-verified PE conjugated anti-SSEA-3 antibody available for identifying, characterizing, and monitoring pluripotency of human ES cells. The MC-631 monoclonal antibody binds to stage-specific embryonic antigen-3 (SSEA-3), a carbohydrate epitope on the major ganglioside, which is present at specific stages during mouse preimplantation embryogenesis. SSEA-3 is expressed on the surface of human teratocarcinoma cells, human embryonic germ (EG) cells, and human ES cells. SSEA-3 is a widely accepted pluripotency marker for human ES cells and is downregulated upon differentiation1-3.

Stemgent® Phycoerythrin (PE) Rat anti-Human SSEA-3 AntibodyProduct Specifications

Immunogen 4-8 cell stage mouse embryos
Reactivity Human
Clone MC-631
Isotype Rat IgM, κ
Number of Tests 50
Formulation Phosphate-buffered solution, pH 7.2, containing ≤0.09% sodium azide.
Preparation This antibody is purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unlabeled antibody.
Storage Store at 4°C. Do not freeze. Light sensitive.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by flow cytometry.
Recommended Usage For flow cytometry, use 20 μl (0.5 μg) per 1x106 viable cells. For a general protocol for performing FC directly on ES cells, see the Technical Documents on the Stemgent® Phycoerythrin (PE) Rat anti-Human SSEA-3 Antibody Product Page at www.stemgent.com/cat#09-0044.
Stemgent® Phycoerythrin (PE) Rat anti-Human SSEA-3 AntibodyReferences
Kannagi, R., Cochran, N.A., Ishigami, F., Hakomori, S., Andrews, P.W., Knowles, B.B., Solter, D. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. The EMBO J. 2: 2355-2361.
Henderson, J.K., Draper, J.S., Baillie, H.S., Fishel, S., Thomson, J.A., Moore, H., Andrews, P.W. (2002) Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens. Stem Cells 20: 329-337.
Brimble, S.N., Sherrer, E.S., Uhl, E.W., Wang, E., Kelly, S., Merrill, A.H. Jr., Robins, A.J., Schulz, T.C. (2007) The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotency. Stem Cells 25: 54-62.
Notice to Purchaser Purchaser represents and warrants that it will use the Stemgent® Phycoerythrin (PE) Rat anti-Human SSEA-3 Antibody purely for research purposes, not for diagnostic use or resale. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Stemgent® Phycoerythrin (PE) Rat anti-Human SSEA-3 Antibody
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