相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 形态:
液态/粉状
- 保存条件:
4℃保存
- 克隆性:
多克隆
- 标记物:
见说明书
- 适应物种:
人,大鼠,小鼠,兔
- 宿主:
Goat,Rabbit,Mouse
- 应用范围:
科研使用
- 浓度:
1mg/1ml
- 靶点:
来电咨询
Product Overview
Stemgent ® StainAlive™ Dylight 488™ Mouse anti-Human TRA-1-81 Antibody was screened on live, unfixed mouse embryonic stem (ES) cells using immunocytochemistry (ICC). This antibody was selected as the best conjugated TRA-1-81 antibody available for visualization of EC/ES/iPS cells, in fluorescent staining and real-time imaging without fixation or killing the cells. This antibody format is useful for direct visualization of the reprogramming process to iPS colonies. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide and is confirmed to be free of bacteria, fungi , mouse virus and mycoplasma as tested. The live ES cell morphology, proliferation and expression of pluripotency markers did not change after staining with this antibody. The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. As human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 AntibodyProduct Specifications
Size / Quantity 50 µg (100 µl at 0.5 mg/ml)
Immunogen Human embryonal carcinoma cell line 2102Ep
Reactivity Human
Clone TRA-1-81
Isotype Mouse IgM,┃
Formulation Phosphate-buffered solution, pH 7.2, containing no sodium azide and 0.2 μm filter sterilized. Endotoxin level is < 1 EU/μg of the protein (< 0.1 ng/μg ) as determined by the LAL assay. It is free from bacteria, fungi, mouse virus and mycoplasma as tested.
Preparation The antibody was purified by affinity chromatography, and conjugated with Dylight™ 488 under optimal conditions. The solution is free of unconjugated dye and free antibody.
Storage and Handling Store at 4°C. Do not freeze. Light sensitive. This formulation contains no preservative. Handle under aseptic conditions.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested in immunocytochemistry (Figure 1) to ensure product quality.
Recommended Usage Use antibody at 1:100 in ICC. For general protocols for performing viable cell immunocytochemistry directly on stem cells, see the Technical Documents on the Stemgent® StainAlive™ Dylight™ 488 anti-Human TRA-1-81 product page at www.stemgent.com/cat#09-0069.
Related Products
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-SSEA-1 Antibody (Cat. # 09-0067)
Notice to Purchaser Purchaser represents and warrants that it will use Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 Antibody purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Trademark DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 Antibody
xy-11304R RFC1复制激活因子C1抗体
xy-11305R RFC2复制激活因子C2抗体
xy-11273R REAB淋巴细胞受体相关蛋白抗体
xy-3023R phospho-RUNX1 (Ser249)磷酸化急性髓细胞白血病1蛋白抗体
xy-3551R RIPK3受体结合丝氨酸苏氨酸激酶3抗体
xym-0855M Insulin (1G11)胰岛素单克隆抗体
xy-3558R phospho-Rb (Ser249)磷酸化视网膜母细胞瘤相关蛋白1抗体
xy-3378R Phospho-Rb (Ser608)磷酸化视网膜母细胞瘤相关蛋白1抗体
xy-9452R RGS4精神分裂症相关蛋白9抗体
xy-6391R RPRD1A细胞周期依赖性激酶抑制相关蛋白抗体
xy-7682R RBM38RNA结合蛋白38抗体
xy-7692R Relaxin 1松弛肽/松弛素抗体
xy-7693R Renalase肾胺酶抗体
xy-9243R RNF20环指蛋白20抗体
xy-9322R RanBP1RAN结合蛋白1抗体
xy-3791R RAE1mRNA结合蛋白抗体
xy-2125R RNF126环指蛋白126抗体
xym-2025M RBC(G2H4)小鼠抗人红细胞单克隆抗体
xy-1501R TIG2视黄酸受体应答蛋白2抗体
xy-1504R RAP1A抗Rap1A抗体
xy-6703R RAB7RAS癌基因相关蛋白RAB7抗体
xy-7827R RCLc-Myc应答蛋白抗体
xy-8307R RAB6BRAS癌基因相关蛋白RAB6B抗体
xy-7826R RABGAP1Rab蛋白GTP酶激活蛋白1抗体
xy-5774R RELM beta结肠癌相关基因蛋白抗体(resistin-like β)
xy-5490R phospho-Ron(Tyr1238)磷酸化原癌基因c-Met相关酪氨酸激酶抗体
xy-9172R RNF6环指蛋白6抗体
xy-5972R Ribonuclease 6核糖核酸酶6抗体
xy-5683R Phospho-RASGRF1 (Ser929)磷酸化Ras特异性鸟嘌呤核苷酸释放因子1
xy-5657R phospho-RAD9(Ser380)磷酸化细胞周期检查控制蛋白质抗体
xy-5658R phospho-RAD9(Ser387)磷酸化细胞周期检查控制蛋白质抗体
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 Antibodyxy-5659R phospho-RAD9(Tyr28)磷酸化细胞周期检查控制蛋白质抗体
xy-5645R phospho-c-Raf(Ser301)磷酸化原癌基因c-Raf抗体
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Vybrant® DyeCycle Green and Orange Stains
degradation. Incubate at 37?C for 30 minutes, protected from light. For cells stained with Vybrant® DyeCycle™ Green stain, analyze samples on flow cytometer using 488 nm excitation and green emission (Figure 3). For cells stained with Vybrant®
together to calculate compensation in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1) Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ Anti-Mouse Bead Kit
Atlas™cDNA表达距阵(Expression Array)
P标记。二者的区别:33P探针杂交图象质量很高,但是价格昂贵,而且灵敏度低;32P却非常适于低丰度基因表达的检测。近年来,Clontech公司不断推出新研究热点的cDNA Array,您可以通过其公司网址上网查询有关资料,也可以向ebiotrade.com客户服务部索取。Atlas™ Human cDNA Expression Array Cat#7740-1人类功能基因组研究的有力工具包括许多在不同生命过程中起关键作用的基因结果信息量大588种人类cDNA点于一带正电的尼龙膜上,另外还包括9个管家
技术资料暂无技术资料 索取技术资料




